摘要
背景:研究表明软骨细胞外基质合成的变化反映了外力对于颞下颌关节的影响和机体对于外力的适应性。目的:观察周期性张应力对髁突软骨细胞主要细胞外基质合成的影响。方法:采用FX-5000T应力加载系统对第3代大鼠髁突软骨细胞分别施加0,1,6,12和24h的周期性张应力,应力刺激强度为10%1Hz。加力完成后收集加力细胞,提取总RNA反转录成cDNA,应用RT-PCR技术检测软骨细胞主要细胞外基质Ⅱ型胶原和聚集蛋白聚糖的表达变化情况。结果与结论:与对照组(0h组)相比,加力6h时Ⅱ型胶原和聚集蛋白聚糖表达均显著增加(P<0.05);加力12h时Ⅱ型胶原和聚集蛋白聚糖表达均开始下降;当加力至24h时二者表达量均显著降低(P<0.05)。结果表明:周期性张应力可以影响髁突软骨细胞主要细胞外基质的合成,随加力时间的延长基质合成逐渐增强;进一步延长加力时间,基质的合成受到明显抑制。
BACKGROUND： Changes in extracellular of chondrocyte can reflect influence of external force on temporomandibular joint and adaptability of body to external force. OBJECTIVE： To study the effect of cyclic tensile stress on main extracellular matrix of condylar chondrocyte. METHODS： The cyclic tensile stress was exposed to the third passage condylar chondrocyte for 0, 1, 6, 12 and 24 hours, respectively, using a Flexcell Strain Unit-5000T system （10% surface elongation, 6 cycles/min）. After mechanical loading, total RNA was extracted from the cells harvested from Six-well BioFlex flexible cell Petri Dish, reverse transcribed, and reverse trabscription-PCR was performed to quantify mRNA levels for type Ⅱ collagen and aggrecan. RESULTS AND CONCLUSION： Compared with the control group （0 hour group）, both type Ⅱ collagen and aggrecan mRNA expression was significantly increased after loading for 6 hours （P 〈 0.05）, but began to decrease since 12 hours, and significantly decreased at 24 hours （P 〈 0.05）. Results showed that cyclical tensile stress stimuli can affect the synthesis of main extracellular matrix of condylar cartilage, i.e. the synthesis was gradually enhanced with prolonged stimulation duration, but significantly inhibited in response to further stress stimuli.
出处
《中国组织工程研究》
CAS
CSCD
2012年第20期3649-3653,共5页
Chinese Journal of Tissue Engineering Research
基金
the National Natural Science Foundation of China,No.31170891~~
