摘要
目的在心肌缺血，再灌注损伤（ischemia／reperfusioninjury，I／RD大鼠探讨磷脂酰肌醇3激酶／丝苏氨酸蛋白激酶（phosphoinositide3kinase／serine-threoninekinase，P13K／Akt）信号转导通路在芬太尼后处理和远隔缺血后处理心肌保护中的作用。方法将32只成年雄性sD大鼠（体重250g-350g）麻醉后，采用计算机产生的随机数随机分为4组：对照组（c组）、芬太尼后处理组（F组）、肢体远隔缺血后处理组（R组）及联合应用芬太尼后处理和肢体远隔缺血后处理组（F_R组）。在结扎大鼠冠状动脉左前降支（1eftanteriordescendingcoronaryartery，LAD）30min造成局部心肌缺血后，开放心肌再灌注60min建立大鼠心肌I／RI模型。采用SABioscience公司功能分类基因芯片和免疫蛋白印迹分析法检测再灌注60min后缺血区心肌内与P13K／Akt相关基因的表达和磷酸化Akt蛋白的表达情况。结果利用基因芯片检测的与P13K／Akt相关的基因中，与C组比较，F组共有9个基因的表达显著上调，而R组仅2个基因的表达显著上调；但F-R组共有33个基因的表达较C组显著上调。蛋白印记分析结果显示，与C组比较，F组、R组和F-R组心肌标本内磷酸化Akt蛋白表达量均增高；而与F组和R组比较，F-R组心肌标本内磷酸化Akt蛋白表达量进一步增高。结论联合应用芬太尼后处理和肢体远隔缺血后处理可明显增强P13K／Akt信号转导通路激活。
Objective To assess the roles of phosphoinositide 3 kinase/serine-threonine kinase （PI3K/Akt） signal pathway in cardioprotection of fentanyl postconditioning and limb remote postconditioning in an in vivo rat model with myocardial ischemia reperfusion injury. Methods Thirty-two anesthetized male SD rats （weighed 250 g-350 g） were randomly allocated into the four groups： group C （control）, group F （fentanyl postconditioning）, group R （RIPOC）, and group F-R （combined fentanyl postconditioning and RIPOC）. All rats were treated with left anterior descending coronary artery（LAD） occluded for 30 rain, followed by a 60-rain reperfusion （LAD open） in vivo. The Olingo PI3k/Akt signaling pathway microarray （SA Bioscience）and Western-blot technique were used to distinguish the expression of genes related to PI3K/Akt pathway and levels of phosphorylated Akt in myocardial tissue sample from ischemia area. Results Expressions of many detected genes relating to PI3K/Akt signal pathway were significantly different among the four groups. As compared with group C, 9 genes showed a significant up-regulated expression in group F, 2 genes showed a significant up-regulated expression in group R, and a total number of 33 genes showed a significant up-regulated expression in group F-R. The Western-blot analysis revealed that the expression of phosphorylated Akt in myocardium increased significantly in groups F, R and F-R compared with group C. The expression of phosphorylated Akt in myocardium was also stronger in group F-R than in groups F and R. Conclusions Combined fentanyl postconditioning and RIPOC can produce an enhanced activation of PI3K/Akt signal pathway.
出处
《国际麻醉学与复苏杂志》
CAS
2012年第6期363-368,共6页
International Journal of Anesthesiology and Resuscitation
