摘要
目的探讨小凹蛋白1（CAV-1）在人皮下和网膜脂肪组织的差异表达，并分析其与肥胖及胰岛素抵抗的关系。方法2009年1至4月在中山大学附属第三医院择期行腹部手术的正常糖调节患者41例，按体质指数（BMI）分为超重组（12例）和正常体重组（29例），采用化学发光法检测两组患者空腹胰岛素（FINS）水平，葡萄糖氧化酶法检测患者空腹血糖（FPG）水平，计算稳态模型的胰岛素抵抗指数（HOMA．IR）；分别在术中取其皮下和网膜脂肪组织，用半定量反转录（RT）-PCR和Western印迹法检测脂肪组织中CAV．1mRNA和蛋白的表达，并对两组患者皮下和网膜脂肪组织中CAV-1mRNA和蛋白表达水平与BMI、HOMA-IR行相关性分析。结果（1）超重组患者血清FINS及HOMA-IR均高于正常体重组[FINS：（8．82±3．79）mU／L比（6．43±4．38）mU／L，P〈0．05；HOMA-IR：（1．91±0．85比1．36±0．72），均P〈0．05]。（2）正常体重组网膜脂肪组织CAV一1mRNA的表达高于超重组（2．84±0．86比1．02±0．36，P〈0．01），但皮下脂肪组织CAV-1mRNA的表达在两组之间差异无统计学意义（1．93±0．62比1．67±0．73，P〉0．05）。（3）正常体重组网膜脂肪组织CAV-1蛋白表达明显高于超重组（1．68±0．67比0．73±0．29，P〈0．05），而在皮下脂肪组织的表达两组之间差异无统计学意义（P〉0．05）。（4）网膜脂肪组织CAV一1mRNA表达水平与BMI、腰围、甘油三酯、HOMA．IR均呈负相关（均P〈0．05）。而皮下脂肪组织CAV-1mRNA表达水平与BMI、腰围及HOMA．IR无明显相关关系（均P〉0．05）。结论CAV-1在肥胖和正常体重者的网膜脂肪组织中存在差异表达，CAV-1在网膜脂肪组织的表达下调可能参与肥胖和胰岛素抵抗的发生。
Objective To explore the depot-specific mRNA and protein expression of caveolin-1 （ CAV-1 ） in human subcutaneous and omental adipose tissues and analyze their relationship with obesity and insulin resistance. Methods A total of 41 subjects with normal glucose regulation were recruited. Among them, there were 29 cases with normal body mass index （BMI） and 12 cases with BMI ≥ 24 kg/m2. All were scheduled to undergone selective abdominal operations. The levels of fasting insulin （FINS） were measured by chemiluminescence enzyme-linked immunosorbent assay （ELISA） kit. Fasting plasma glucose （FPG） was tested by glucose oxidase and home model insulin resistance index （I-IOMA-IR） calculated. Real-time polymerase chain reaction （RT-PCR） and Western blotting were employed to assess the relative mRNA and protein expression of caveolin-1 in subcutaneous and omental adipose tissues. And the mRNA and protein expression of caveolin-1 from different fat depots were compared and their correlations with BMI and HOMA-IR analyzed. Results （1） FINS and HOMA-IR were significantly higher in the overweight and obesity group than those in the normal BMI group （FINS： （8.82±3.79） mU/L vs （6.43±4. 38） mU/L, P 〈 0. 05, HOMA-1R： 1.91 ±0. 85 vs 1.36 ± 0. 72, P 〈 0.05 ）. （2） The normal BMI group patients had the higher expression levels of caveolin-1 mRNA in omental adipose tissue than overweight counterparts （2. 84±0. 86 vs 1.02 ± 0. 36, P 〈 0. 01 ）. But the difference in subcutaneous adipose tissue was not significant （P 〉0. 05 ）. （3）The caveolin-1 protein expression in omental adipose tissue of the normal BMI group was higher than that of overweight patients （ 1.68 ± 0. 67 vs 0. 75 ± 0. 29, P 〈 0.05 ）. And the difference between two groups was not significant （ P 〉 0. 05 ）. （4） The expressions of caveolin-1 mRNA in omental adipose tissue were negatively correlated with BMI, waist circumstance, triglyceride and HOMA-IR （r = - 0. 441, - 0. 615,
出处
《中华医学杂志》
CAS
CSCD
北大核心
2012年第20期1388-1391,共4页
National Medical Journal of China
基金
国家自然科学基金（81070661/H0713）
