摘要
目的观察人脐带间充质干细胞(human umbilical cord mesenchymal stem cell,hUC-MSC)与聚吡咯共培养后,在不同通电条件下形态学变化。方法体外分离培养hUC-MSC并进行鉴定。循环伏安法制备聚吡咯(polypyrrole,PPy)薄膜。选择第3～6代的hUC-MSC与PPy共培养,分别采用控制电压和控制电流的方法对细胞进行刺激,利用电镜及光镜观察细胞形态学变化。不通电组作为阴性对照。结果电刺激对细胞贴附、形态有较大影响,不同形式的电刺激对细胞形态影响的比较类似。结论电刺激结合生物导电材料对细胞的贴附和形态可造成显著改变,可作为调控细胞生长的手段之一,需要进一步研究其控制条件。
Objective To observe the morphology change of human umbilical cord mesenchymal stem cell（hUC-MSC） cultured on polypyrrole（PPy） under the conditions of different styles of electric stimuli.Method hUC-MSC were identified by flow cytometry and inverted phase contrast microscope.Passage 3 to 6 hUC-MSC were co-cultured with PPy.Current-controlled and voltage-controlled electric stimuli（ES） were applied to the cells.Cells cultured in αMEM with 10% fetal bovine serum and cells cultured on PPy without ES were used as control.Scan electron microscope（SEM） and inverted phase contrast microscope were used to observe the morphology change of hUC-MSC.Result The effect of ES on the adhesion and morphology change of hUC-MSC was evident and similar in different groups.Conclusion ES has distinct effect on hUC-MSC adhesion and morphology and can be used as a method of cell regulation.More research are needed.
出处
《中国比较医学杂志》
CAS
2012年第5期14-17,80,共5页
Chinese Journal of Comparative Medicine
基金
北京市自然科学基金资助项目(2112017)
国家863"干细胞与组织工程"重大项目(2006AA02A115863)
关键词
间充质干细胞
聚吡咯
组织工程学
电刺激
Mesenchymal stem cell
Polyppyrole
Tissue engineering
Electric stimulus
