摘要
本研究利用电子克隆的方法获得花生转录因子WRI1的cDNA序列(AhWRI1),采用生物信息学方法,预测和分析AhW RI1的序列特点、编码蛋白AhW RI1的特性以及与其他植物氨基酸序列的相似性。结果表明:AhW RI1含一个长度为780bp的完整开放阅读框架,编码259个氨基酸。编码蛋白AhWRI1包含2个典型的AP2功能域,是亲水性蛋白,在蛋白质的三级结构上与拟南芥和油菜的WRI1相似。AhW RI1与拟南芥、油菜WRI1氨基酸保守序列同源性在81.87%～100%之间。AhW RI1无序化程度为71.8%,比拟南芥低3.8%,比油菜高2.5%。亚细胞定位显示AhW RI1在细胞核内,并预测该蛋白具有转录复制,调控及转录与结合的可能性分别为0.244、0.226和0.152。研究结果为花生WRI1基因的分子克隆,功能鉴定提供理论基础。
The cDNA sequence of transcription factor WRI1 （AhlVRH） from peanut was obtained through electronic cloning method. The features of cDNA sequence, the properties of AhWRI1 protein as well as its similarities with the amino acid sequences of other plants were predicted and analyzed with bioinformatics methods. Results show that AhWRI1 gene has a full length open reading frame of 780 bp encoding 259 amino acids, and the relevant protein is hydrophilic and has two typical AP2 domains and shows high similarity in tertiary structure with the WRI1 from Arabidopsis and Brassica. The amino acids conserved sequence of AhWRI1 is 81.87%-100% homologous with that of Arabidopsis and Brassica. The degree of disordering of AhWRIlis 71.8%, 3.8% lower than that ofA rabidopsis thaliana, while 2.5% higher than that of Brassica. Subcellular localization of AhWRI1 reveals that this protein lies in the nucleus, and the possibilities that this protein has the functions of transcription and replication,regulation and transport_and_binding are 0.244, 0.226, 0.152 respectively. The research provides a basis for the molecular cloning and functional identification of WRI1 from peanut.
出处
《分子植物育种》
CAS
CSCD
北大核心
2012年第3期363-370,共8页
Molecular Plant Breeding
基金
山东省花生良种产业化工程项目
现代农业产业技术体系建设专项资金资助项目(CAIS-14)共同资助
