摘要
AIM:To establish a rat ethanol gastritis model,we evaluated the effects of ethanol on gastric mucosa and studied the preventive effects of geranylgeranylacetone on ethanol-induced chronic gastritis.METHODS:One hundred male Sprague-Dawley rats were randomly divided into 4 equal groups:normal control group,undergoing gastric perfusion of normal saline(NS) by gastrogavage;model control group and 2 model therapy groups that underwent gastric perfusion with ethanol(distillate spirits with 56% ethanol content) by gastrogavage for 4 wk.Low or high doses of geranylgeranylacetone were added 1 h before ethanol perfusion in the 2 model therapy groups,while the same amount of NS,instead of geranylgeranylacetone was used in that model control group.The rats were then sacrificed and stomachs were removed.The injury level of the gastric mucosa was observed by light and electron microscopy,and the levels of prostaglandin 2(PGE 2),endothelin-1(ET-1) and nitric oxide(NO) were measured by radioimmunoassay and the Griess method.RESULTS:The gastric mucosal epidermal damage score(EDS;4.5) and ulcer index(UI;12.0) of the model control group were significantly higher than that of the normal control group(0 and 0 respectively,all P = 0.000).The gastric mucosal EDS and UI of the 2 model therapy groups(EDS:2.5 and 2.0;UI:3.5 and 3.0) were significantly lower than that of the model control group(all P < 0.01).There was no statistically significant difference between the low-dose and high-dose model therapy groups.The expression value of plasma ET-1 of the model control group was higher than that of the normal control group(P < 0.01) and the 2 model therapy groups(all P < 0.01).The expression values of gastric mucosal PGE 2 and serum NO of the model control group were lower than those of the normal control group(all P < 0.05) and the 2 model therapy groups(all P < 0.05).The thickness of the gastric mucous layerand the hexosamine content in the model control group were significantly lower than that in the normal control group(all P < 0.01) an
AIM：To establish a rat ethanol gastritis model,we evaluated the effects of ethanol on gastric mucosa and studied the preventive effects of geranylgeranylacetone on ethanol-induced chronic gastritis.METHODS：One hundred male Sprague-Dawley rats were randomly divided into 4 equal groups：normal control group,undergoing gastric perfusion of normal saline（NS） by gastrogavage;model control group and 2 model therapy groups that underwent gastric perfusion with ethanol（distillate spirits with 56% ethanol content） by gastrogavage for 4 wk.Low or high doses of geranylgeranylacetone were added 1 h before ethanol perfusion in the 2 model therapy groups,while the same amount of NS,instead of geranylgeranylacetone was used in that model control group.The rats were then sacrificed and stomachs were removed.The injury level of the gastric mucosa was observed by light and electron microscopy,and the levels of prostaglandin 2（PGE 2）,endothelin-1（ET-1） and nitric oxide（NO） were measured by radioimmunoassay and the Griess method.RESULTS：The gastric mucosal epidermal damage score（EDS;4.5） and ulcer index（UI;12.0） of the model control group were significantly higher than that of the normal control group（0 and 0 respectively,all P = 0.000）.The gastric mucosal EDS and UI of the 2 model therapy groups（EDS：2.5 and 2.0;UI：3.5 and 3.0） were significantly lower than that of the model control group（all P 〈 0.01）.There was no statistically significant difference between the low-dose and high-dose model therapy groups.The expression value of plasma ET-1 of the model control group was higher than that of the normal control group（P 〈 0.01） and the 2 model therapy groups（all P 〈 0.01）.The expression values of gastric mucosal PGE 2 and serum NO of the model control group were lower than those of the normal control group（all P 〈 0.05） and the 2 model therapy groups（all P 〈 0.05）.The thickness of the gastric mucous layerand the hexosamine content in the model control group w
