摘要
目的建立PEG-PLGA纳米粒中银杏内酯B(GB)的包封率和载药量测定的HPLC-ELSD方法。方法色谱条件为:Shiseido C_8柱(250 mm×4.6 mm,5μm);V(甲醇):V(四氢呋喃):V(水)=31:11:58为流动相;蒸发光散射检测器检测参数:蒸发口温度105℃,载气(N_2)流速1.2 L·min^(-1),柱温45℃。应用低温超速离心法和凝胶过滤相结合的方法纯化纳米粒。结果本次建立的HPLC-ELSD方法在GB质量浓度50～500 mg·L^(-1)内线性关系良好,r=0.999 1;重复性试验RSD为4.28%;平均回收率为95%～105%;载GB的PEG-PLGA纳米粒中杂质对GB的测定无干扰。GB的包封率为75.33%,载药量为3.77%。结论该方法简单、快速、准确,可用于载GB的PEG-PLGA纳米粒中GB包封率和载药量的测定。
A/M To establish a HPLC method with evaporative light scattering detector （ELSD） to determine the encapsulating efficiency and loading capacity of ginkgolide B（GB） in poly（ethyleneglycol） -poly（ L-lactic-co-glycolic acid） （PEG-PLGA） nanoparticles. METHODS Chromatographic conditions were as follows： Shiseido C8 column （250 mm ×4.6 mm, 5 μn） ; V（ methanol） ： V（ tetrahydrofuran ）： V（water ） = 31 ： 11：58 as mobile phase. ELSD parameters were set as follows： evaporation temperature was 105℃, carrier gas （N2） flow rate was 1.2 L·min-1 and column temperature was 45℃. Low temperature ultracentrifugation and gel filtration methods were used to purify nanoparticles. RESULTS In the range of 50 - 500 mg·L^-1, all plots were linear and the correlation coefficients were high than 0.999 0; the relative standard deviation （RSD） of the reproducibility was 4.28 % ; average recovery was in the range of 95 % - 105 %. As the dosage of GB was 1 mg, the encapsulating efficiency（EE） and the loading capacity（LC） was 75.33% and 3.77%, respectively. CONCLUSION The method is simple, rapid, accurate and can be used for quality control of GB loaded nanopartieles.
出处
《中国临床药学杂志》
CAS
2012年第3期137-140,共4页
Chinese Journal of Clinical Pharmacy
基金
国家自然科学基金:基于冰片"开窍"与aprotinin修饰的中药有效部位脑靶向纳米给药系统(编号81001642)
关键词
高效液相色谱
蒸发光散射检测
银杏内酯B
PEG-PLGA纳米粒
包封率
载药量
HPLC
evaporative light scattering detection
ginkgolide B
poly（ethyleneglycol） - poly（ L- lactic-co-glycolic acid） nanoparticle
encapsulating efficiency
loading capacity
