摘要
目的检测Cath-D和MMP-9和在皮肤恶性黑色素瘤、交界痣及正常皮肤中表达情况,及其与皮肤恶性黑色素瘤的临床分期,淋巴结转移的关系。方法应用免疫组化S-P法对分别检测Cath-D和MMP-9在44例皮肤恶性黑色素瘤、22例皮肤交界痣及20例正常皮肤中的表达水平。结果 Cath-D和MMP-9在皮肤恶性黑色素瘤中的阳性表达率(79.55%和75.00%)均高于交界痣(45.45%和40.91%)及正常皮肤组织(15.00%和30.00%)(P<0.05),Cath-D和MMP-9在皮肤恶性黑色素瘤中的阳性表达率随肿瘤浸润深度的增加而增高,且有淋巴结转移者阳性表达率大于无淋巴结转移者(P<0.05)。皮肤恶性黑色素瘤中Cath-D和MMP-9的表达呈正相关(rs=0.488,P<0.05)。结论 Cath-D和MMP-9在皮肤恶性黑色素瘤的发生、发展中可能起着重要作用,且与CMM的浸润转移密切相关,且二者之间可能具有协同作用。联合检测两者的表达情况可为CMM的早期诊断及预后提供新的靶点。
Objective To investigate the expression of cathepsin D(cath-D) and matrix metalloproteinase 9(MMP-9) in cutaneous malignant melanoma(CMM),junctional nevus and normal skin,and its relationship with the clinical stages and lymph node metastasis of CMM.Methods The expression of Cath-D and MMP-9 was detected in 44 cases of CMM,22 cases of junctional nevus and 20 cases of normal skin specimens by SP immunohistochemical method.Results The positive expression rates of Cath-D and MMP-9 in the CMM(79.55% and 75.00%) were higher than those in the junctional nevus(45.45% and 40.91%) and normal skin(15.00% and 30.00%)(P<0.05).The positive expression rates of Cath-D and MMP-9 in the CMM increased along with the increase in the depth of tumor invasion,and higher positive expression rates of Cath-D and MMP-9 were detected in the CMM patients with lymph node metastasis than in those without lymph node metastasis.There was a positive correlation between Cath-D and MMP-9 expression in the CMM tissues(rs=0.488,P<0.05).Conclusion Cath-D and MMP-9 are important in the tumorigenesis and development of CMM and are closely related to CMM invasion and metastasis,with a possible synergistic effect.Combined detection of Cath-D and MMP-9 expression may help both early diagnosis and prognosis of CMM.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第10期1002-1005,共4页
Journal of Third Military Medical University
