摘要
为了准确地检测流感病毒唾液酸α2,3-半乳糖和唾液酸α2,6-半乳糖两种受体在人、小鼠、鸡及鸭气管和肺组织中分布和表达的特征,本研究利用凝集素免疫荧光组织染色方法对各种组织切片进行了系统的定性和定量分析.定性分析的结果,显示两种流感病毒唾液酸受体在人、小鼠、鸡及鸭肺组织和小鼠及鸭气管组织中均有表达,但是,唾液酸α2,6-半乳糖受体只在人气管组织中有表达而唾液酸α2,3-半乳糖受体只在鸡气管组织中有表达.定量分析的结果显示,两种流感病毒唾液酸受体在人和小鼠的气管组织以及人、鸡和鸭的肺组织均有表达.同时,本研究结果还显示了流感病毒受体在同一组织中的分布和表达并不是均匀的,表明它们在各种组织中的分布和表达并非简单的有和无之间的区别,而是一种在表达数量上多和少的差别.
To determine precisely the expression and distribution patterns of two influenza virus receptors （SAα2,3-gal and SAα2,6-gal） in trachea and lung tissues of human, mouse, chicken and duck, we analyzed lectin immunofluorescence stainings of various tissue sections qualitatively and quantitatively. Results from the qualitative analysis showed that both influenza virus receptors were expressed in lung tissues of human, mouse, chicken and duck as well as trachea tissues of mouse and duck; however, SAα2,6-gal receptor was expressed only in the human trachea tissue and SAα2,3-gal receptor was expressed only in the chicken trachea tissue. Results from the quantitative analysis demonstrated that both receptors were expressed in trachea tissues of human and mouse, as well as in lung tissues of human, chicken and duck. Meanwhile, our results also showed that the expression and distribution of influenza virus receptors in the same tissue were not always uniform, indicating that their distribution and expression in various tissues are not simply the distinction between the presence or absence of receptors, but rather the difference in the amount of expressed receptors.
出处
《中国科学：生命科学》
CSCD
北大核心
2012年第5期383-389,共7页
Scientia Sinica(Vitae)
基金
贵州省优秀科技教育人才省长专项资金(批准号:黔省专合字2010-68号)资助项目
关键词
流感病毒
唾液酸受体
气管
肺
凝集素
免疫荧光
定量分析
定性分析
influenza virus, siaUc acid receptors, trachea, lung, lectin, immunofluorescence, quantitative analysis, qualitative analysis
