期刊文献+

鸭坦布苏病毒E蛋白的原核表达 被引量:9

EXPRESSION OF E PROTEIN OF DUCK TEMBUSU VIRUS IN PROKARYOTIC CELLS
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摘要 本研究利用RT-PCR方法扩增鸭坦布苏病毒(Duck tembusu virus,DTMUV)奉贤株(FX2010)的结构蛋白E基因,并将其克隆至原核表达载体pET32a(+),构建重组表达质粒pET32a-E。将其转化受体菌E.coli BL21(DE3)感受态细胞,经IPTG诱导进行表达。SDS-PAGE和Western blot试验检测结果表明,E基因在大肠杆菌中得到了表达,并且可与抗DTMUV的多克隆抗体发生特异性反应。E基因的成功表达为DTMUV的诊断试剂盒、疫苗等的研制奠定基础。 The E gene of duck Tembusu virus(DTMUV) was amplified in RT-PCR and inserted into the multiple clone sites of the pET32a(+) vector.The recombinant plasmid was transformed into BL21(DE3) competent cells,and expression of the E protein was induced with IPTG.The E protein was expressed in BL21(DE3) cells and visualized to react with specific DTMUV antibody in SDS-PAGE and Western blot.
出处 《中国动物传染病学报》 CAS 2012年第2期42-46,共5页 Chinese Journal of Animal Infectious Diseases
基金 公益性行业(农业)专项经费(201003012) 国家国际科技合作项目(2010DFB33920) 中国农业科学院上海兽医研究所中央级公益性科研院所基本科研业务费专项资金项目(2010JB04)
关键词 鸭坦布苏病毒 E基因 克隆 表达 Duck Tembusu virus E gene cloning expression
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参考文献13

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共引文献49

同被引文献86

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