摘要
目的以5-溴脱氧尿嘧啶核苷(5-bromodeoxyuridine,5-BrdU)为标记对小鼠喉黏膜可能存在干细胞进行初步研究。方法选取出生3 d的昆明小鼠36只,随机分为A、B和C组,每组12只。A组小鼠皮下注射100μg/g的5-BrdU,B组小鼠皮下注射50μg/g的5-BrdU,C组小鼠皮下注射生理盐水。第8周后分别处死各组小鼠,并取小鼠喉黏膜进行免疫组化检测,计算其标记滞留细胞的阳性率。结果 8周后,注射了5-BrdU的A组与B组小鼠喉黏膜鳞状上皮有标记滞留细胞表达但两组间表达无统计学意义(t=0.06,P=0.949)。C组小鼠喉黏膜鳞状上皮无标记滞留细胞表达。结论 A、B两组小鼠喉黏膜存在标记滞留细胞,此标记滞留细胞具有喉黏膜成体干细胞的一些特点,可能是成体干细胞的来源。
Objective To identify the existence of stem cells in mouse laryngeal mucosa by using 5-bromodeoxyurdine（5-BrdU） labeling for the preliminary study.Methods 36 three-days Kunming white mice were randomly divided into group A,B and C.5-BrdU of 100 μg/g and 50 μg/g were intraperitoneally injected to mice of group A and group B respectively,and normal saline to those of group C.All the mice were executed 8 weeks after injection.Immunohistochemical staining was applied to detect the label-retaining cells（LRCs） in the laryngeal mucosa,and the percentages of LRCs were calculated.Results There were LRCs in scalyepithelium of both group A and B,and the difference between the two groups was statistically insignificant（t=0.06,P=0.949）.The expression of LRCs was not observed in group C.Conclusion There are LRCs in the mouse laryngeal mucosa.It is possible that stem cells exist in these LRCs.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2012年第2期90-93,共4页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
广东省自然科学基金(9151008901000006)
