摘要
背景:低分子肝素和水蛭素都有活血化淤作用。目的:观察局部注射低分子肝素或重组水蛭素对静脉淤血皮瓣成活的影响。方法:在24只兔左耳背制作3cm×6cm静脉淤血皮瓣模型,随机分组:对照组皮瓣下多点注射生理盐水;肝素组皮瓣下多点注射低分子肝素625U/mL,水蛭素组皮瓣下多点注射重组水蛭素1U/mL。结果与结论:①大体观察:术后水蛭素组和肝素组较对照组淤血程度轻,血肿消散快。②皮瓣成活面积百分比:水蛭素组、肝素组明显高于对照组(P<0.01),水蛭素组和肝素组比较差异无显著性意义(P>0.05)。③苏木精-伊红染色结果:对照组术后同期微血管淤血程度较水蛭素组和肝素组严重,微血管计数明显少于水蛭素组和肝素组(P<0.05,P<0.01),水蛭素组微血管计数明显高于肝素组(P<0.01)。说明局部应用低分子肝素或重组水蛭素可较快改善皮瓣的淤血,提高皮瓣成活,重组水蛭素效果优于低分子肝素。
BACKGROUND：Low-molecular-weight heparin（LH） and recombinant hirudin（RH） can promote blood circulation.OBJECTIVE：To investigate the effect of local injection of LH or RH on the survival of venous congested skin flap.METHODS：Twenty-four rabbits were employed to use the 3 cm×6 cm venous congested skin flap model on the back of left ear.The models were randomly divided into three groups：the control group was performed with multi-point injection of saline under skin flap;LH group was performed with multi-point injection of 625 U/mL LH under skin flap;RH group was multi-point injected with 1 U/mL RH under skin flap.RESULTS AND CONCLUSION：① General observation showed that the congestion in RH group and LH group was lighter than that in control group,and the hematoma dissipate quickly in RH group and LH group.② The percentage of flap survival area in RH group and LH group was higher than that in the control group obviously（P 0.01）,there was no significant difference between RH group and LH group（P 0.05）.③ Hematoxylin-eosin staining showed that congestion of microvascular in control group after operation was more serious than that in the RH group and LH group（P 0.05,P 0.01）,the number of microvascular in RH group was higher than that in the LH group（P 0.01）.Local application of LH and RH can improve the congestion of skin flap,inhibit the survival rate of flap.There was better effect of using RH than LH.
出处
《中国组织工程研究》
CAS
CSCD
2012年第18期3329-3332,共4页
Chinese Journal of Tissue Engineering Research
