摘要
目的探讨不同浓度苦参碱对神经母细胞瘤SH-SY5Y细胞存活及凋亡的影响,及苦参碱降低顺铂化疗耐药性的作用及可能机制。方法分别以全反式维甲酸(ATRA)、脑源性神经生长因子(BDNF)、顺铂、苦参碱处理SH-SY5Y细胞,实验分对照组、ATRA诱导的顺铂干预组、顺铂干预组、苦参碱干预组、苦参碱联合顺铂干预组。应用四甲基偶氮蓝比色法检测苦参碱与顺铂对SH-SY5Y细胞存活率的影响,流式细胞仪检测苦参碱与顺铂对SH-SY5Y细胞凋亡率的影响,Western-blot方法检测SH-SY5Y细胞的p-TrkB表达。结果除ATRA诱导的顺铂干预组外,其余各组SH-SY5Y细胞的存活率及凋亡率与对照组比较,差异均有统计学意义(P<0.05);顺铂干预组与ATRA诱导的顺铂干预组比较,SH-SY5Y细胞的存活率及凋亡率差异有统计学意义(P<0.05);相同浓度的苦参碱干预组与苦参碱联合顺铂干预组比较,SH-SY5Y细胞的存活率及凋亡率差异有统计学意义(P<0.05);苦参碱联合顺铂组与顺铂干预组比较,SH-SY5Y细胞的存活率及凋亡率差异有统计学意义(P<0.05)。苦参碱0.5 mg/ml组与顺铂干预组比较,SH-SY5Y细胞的存活率及凋亡率差异无统计学意义(P>0.05)。各组SH-SY5Y细胞间,p-TrkB的表达水平差异无统计学意义(P>0.05)。结论苦参碱可以抑制SH-SY5Y细胞的增殖并诱导凋亡,从而提高顺铂化疗的敏感性,该作用与TrkB/BDNF信号通路(间)无相关性。
Objective To explore the effects of matrine（Ma） on survival and apoptosis of neuroblastoma cell line SH-SY5Y,reduction of drug-resistance of cisplatin（CDDP） and its potential mechanism.Methods SH-SY5Y cells were treated with all-trans-retinoic acid（ATRA）,brain-derived neurotrophic factor,CDDP and Ma respectively.Trials were divided into following groups： control group,ATRA induced cisplatin intervention group,cisplatin intervention group,Ma intervention group,and Ma combined with cisplatin intervention group.MTT colorimetric assay was performed to exam the survival rate of SH-SY5Y cells treated with Ma and CDDP.Apoptosis rate of SY5Y was measured by flow cytometry Ma.Western-blot analysis was employed to measure the p-TrkB expression.Results There was significant difference in survival and apoptosis of SH-SY5Y cells between each group and control group except the ATRA induced cisplatin intervention group（P 0.05）.Significant difference was also found between cisplatin intervention group and ATRA induced cisplatin intervention group（P 0.05）.The survival rates and apoptosis of SH-SY5Y cells was statistically significant between Ma group and Ma combined with cisplation group（P 0.05）;and also between the Ma joint cisplation group and cisplation intervention group（P 0.05）;There was no significant difference in survival and apoptosis of SH-SY5Y cells between Ma（0.5 mg / ml） intervention group and ATRA induced cisplatin group（P 0.05）.No significant difference was observed in p-TrkB expression of SH-SY5Y cells among groups（P 0.05）.Conclusions Ma can induce cell apoptosis and inhibit the growth of the SH-SY5Y cells,meanwhile increase the sensibility of chemotherapy of cisplatin but this effect is not correlated to the TrkB /BDNF signal transduction.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2012年第5期456-459,共4页
Journal of Clinical Pediatrics
