摘要
目的观察电针对脑心综合征(cerebral-cardiac syndrome,CCS)大鼠血肿周围脑组织细胞凋亡及Caspase-3表达的影响。方法从70只健康SD大鼠中随机选择10只作为假手术组,其余60只用于CCS模型复制。选取造模成功的大鼠30只,分为模型组、电针组和电针非经非穴组,每组10只。采用胶原酶加肝素联合注射大鼠尾状核方法造模,假手术组向大鼠尾状核注入等量0.9%氯化钠注射液。于造模第1天开始电针,电针组选取水沟、风府、内关和心俞穴,电针非经非穴组选取臀部非经非穴点4处,每次20min,每天1次,连续3天。假手术组和模型组仅每天抓空1次。分别采用TUNEL法和免疫组化法检测血肿周围脑组织细胞凋亡及Caspase-3表达。结果假手术组偶可见TUNEL细胞,Caspase-3少量表达;模型组血肿周围组织出现较多的TUNEL阳性细胞,Caspase-3大量表达;电针组TUNEL阳性细胞和Caspase-3表达均较模型组和电针非经非穴组明显减少(P<0.01)。结论电针可以抑制CCS大鼠脑组织细胞凋亡,其机制可能与下调血肿周围脑组织Caspase-3表达有关。
Objective To observe the effects of electroacupuncture （EA） on the apoptosis of brain tissue cells and the expression of Caspase-3 in the rats with cerebral-cardiac syndrome （CCS）. Methods A total of 70 healthy SD rats were selected. Ten was randomly recruited as the sham-operation group, and the rest were used for CCS model preparation. Thirty successfully modeled rats were divided into the model group, the EA group, and the non-EA group, 10 in each group. The model was prepared using injecting collagenase ＋ heparin into the caudate nucleus. Equal volume of 0. 9% sodium chloride injection was injected to rats＇ caudate nucleus in the sham-operation group. EA was started on the 1st day of modeling. Shuigou （GV26）, Fengfu （GV16）, Neiguan （PC6）, and Xinshu （BL15） were needled in the EA group. Four points in the hips were needled in the non-EA group. The EA needling lasted for 20 min each time, once daily, for 3 successive times. No EA was administered to the sham- operation group or the model group. The apoptosis of brain tissue around the hematoma and the expression of Caspase-3 were detected using TUNEL and immunochemical assay. Results TUNEL cells could be occasionally seen with fewer Caspase-3 expression in the sham-operation group. More TUNEL positive cells appeared in the tis- sue around the hematoma of the model group with a large amount of Caspase-3 expression. The TUNEL positive cells and Caspase-3 expression were obviously less in the EA group than in the model group and the non-EA group （P 〈0. 01 ）. Conclusions EA could inhibit the apoptosis of brain tissue cells in CCS rats. Its mechanisms might be associated with down-regulating the Caspase-3 expression of the brain tissue around the hematoma.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2012年第5期639-642,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
安徽省自然科学基金项目(No.11040606M188)
安徽省人才开发专项资金项目(No.2008Z036)
安徽中医学院自然科学研究项目(No.2011zr009B)
关键词
电针
脑心综合征
细胞凋亡
半胱氨酸天冬氨酸蛋白酶3
electroacupuncture
cerebral-cardiac syndrome
cell apoptosis
cysteine containing aspartate specific protease-3
