摘要
目的探讨大肠癌中医证型与核苷酸切除修复交叉互补基因1(excision repair cross-complementing 1,ERCC1)C8092A和C19007T两个位点基因多态性的关系。方法 99例大肠癌患者经中医辨证分为湿热蕴结、气滞血瘀、脾肾阳虚及肝肾阴虚4个证型。应用PCR扩增和直接测序法检测患者外周静脉血ERCC1C8092A和C19007T两个位点基因型及等位基因在大肠癌各证型的分布情况,并进行统计学分析。结果 ERCC1C8092A基因型及等位基因频率在各证型间的分布比较,差异均无统计学意义(P>0.05)。ERCC1C19007T基因型及等位基因频率在各证型间的分布频率比较,差异有统计学意义(P<0.05),其中湿热蕴结型与气滞血瘀型、脾肾阳虚型与肝肾阴虚型差异无统计学意义(P>0.05),湿热蕴结型与脾肾阳虚型、肝肾阴虚型差异有统计学意义(P<0.05),气滞血瘀型与脾肾阳虚型、肝肾阴虚型差异有统计学意义(P<0.05)。结论 ERCC1C19007T基因多态性可能与大肠癌中医证型有关,需要进一步研究。
Objective To explore the correlation between excision repair cross-complementing 1 （ERCC1） C8092A and C19007T gene polymorphisms and different Chinese medicine （CM） syndrome types of colorectal cancer （CC）. Methods Ninety-nine patients with CC were syndrome typed as dampness-heat accumulation syndrome, qi stagnation with blood stasis syndrome, Pi-Shen yang deficiency syndrome, and Gan-Shen yin deficiency syndrome. The gene polymorphisms of excision repair cross-complementing 1 （ ERCC1 ） C8092A and C19007T in different CM syndrome types of CC were examined by polymorphisms chain reaction amplification and direct sequencing, and analyzed statistically. Results The frequencies of C8092A genotype and allele in different CM syndrome types had no statistical difference （P〉0. 05）. The frequencies of C19007T genotype and allele in different CM syndrome types had statistical difference （P〈0. 05）. Of them, there was no statistical difference in the frequencies between dampness-heat accumulation syndrome and qi stagnation with blood stasis syndrome, or between Pi-Shen yang deficiency syndrome and Gan-Shen yin deficiency syndrome （P〉0. 05）. There was statistical difference between dampness-heat accumulation syn-drome and Pi-Shen yang deficiency syndrome as well as Gan-Shen yin deficiency syndrome （ P 〈0. 05）. There was statistical difference between qi stagnation with blood stasis syndrome and Pi-Shen yang deficiency syndrome as well as Gan-Shen yin deficiency syndrome （P 〈0. 05）. Conclusion ERCC1 C19007T gene polymorphisms might be associated with CM syndrome types of CC, which needed to be further studied.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2012年第5期628-632,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
福建省科学技术厅资助项目(No.2006Y0010)
关键词
大肠癌
中医证型
核苷酸切除修复交叉互补基因1
基因多态性
colorectal cancer
Chinese medicine syndrome type
excision repair cross-complementing 1
gene polymorphism
