摘要
优化了塔玛亚历山大藻Alexandrium tamarense CI01细胞同步化方法,研究了N、P限制对A.tamarense CI01细胞周期及毒素含量和组成变化的影响。结果表明,采用前期驯化、细胞沉降分离和光限制诱导相结合的同步化方法,细胞同步率提高了约14%。N、P限制条件下,细胞分裂受抑制,分别停滞于G2/M期和G1期。营养盐对毒素合成及组成影响比较明显:N限制下单细胞毒素含量降低,P限制下单细胞毒素含量升高。在正常营养和P限制条件下,C1,2占毒素总量的98%以上,GTX2,3占总毒素量的2%左右;N限制条件下,C1,2所占比例下降到90%左右,而GTX2,3比例增长到约10%。根据C1,2和GTX2,3在一个细胞周期内的变化规律推测,毒素的合成受光诱导并在细胞周期G1期的特定时间内合成,细胞可能先合成GTX2,3毒素,后被转化为C1,2毒素。
This study optimized the cell synchronized method of Alexandrium tamarense CI01 and investigated the effects of nutrient limitation on cell cycle, toxin content and composition of A. tamarense CI01. The result showed that the synchronization ratio of A. tarnarense CI01 cells increased 14% compared to the traditional darkness induced method. Under nutrient limitation conditions, ni- trate-limited cells were arrested at G2/M phase while phosphate-limited cells were arrested at G1 phase. Toxin content and composi- tion of A. tamarense CI01 varied significantly at the different cell cycle phases. Nitrate-limitation decreased toxin content while phos- phate-limitation increased toxin content compared to the nutrient replete condition. Compared to nutrient replete and phosphate-limita- tion conditions, the ratio of C1,2declined from 98% to 90% , and the ratio of GTX2,3 increased from 2% to 10% under nitrate-limita- tion. Toxin biosynthesis was induced by light and always occurred during a defined time frame within the G1 phase of the cell cycle. Analyzing toxin biosynthesis time and ratios of C1,2 and GTX2,3 toxins in cells, we postulate that GTX2,3 is synthesized firstly and then is transferred to C1.2 in A. tamarense CI01.
出处
《海洋环境科学》
CAS
CSCD
北大核心
2012年第3期352-357,共6页
Marine Environmental Science
基金
国家自然科学基金(40776068,40876059)
