摘要
目的研究大黄素对急性坏死性胰腺炎(Acute necrotizing pancreatitis,ANP)大鼠是否有保护作用。方法 40只健康雄性SD大鼠随机分为4组,对照组、ANP组、大黄素预防组,大黄素治疗组。大剂量精氨酸腹腔注射诱导ANP模型,预防组于造模前90min给予大黄素25mg/kg,两次,每次间隔1h,治疗组于造模后30min给予大黄素25mg/kg,两次,每次间隔1h。各组大鼠于造模后24h处死,观察大鼠血浆肾素、血管紧张素Ⅱ(AngiotensinⅡ,AngⅡ)、AMY、TNF-α、IL-1β水平变化,并检测胰腺/体重比值、胰腺病理变化。结果 ANP组肾素、AngⅡ、AMY、TNF-α、IL-1β、胰腺/体重比值均较对照组明显升高;胰腺组织广泛坏死,弥漫性水肿及大量炎细胞浸润,胰腺小叶结构破坏严重。大黄素预防组和治疗组血浆AMY、TNF-α、IL-1β、胰腺/体重比值较ANP组明显下降,胰腺组织病理损伤明显减轻,而血浆肾素、AngⅡ水平较ANP组变化不明显。结论大黄素对血浆肾素、AngⅡ水平无明显影响,但可减少AMY、TNF-α、IL-1β的产生,减轻胰腺组织的病理损伤,对ANP损伤具有保护作用。
Objective To investigate the effect of emodin on acute necrotizing pancreatitis（ANP） in rats.Methods A total of 40 Sprague-Dawley rats were randomized into 4 groups（n=10） including control group,ANP group,prevention group and emodin-treated group.ANP was induced in rats by intraperitoneal infusion of large doses of arginine.Emodin was given twice,each 1 hour interval,90 minutes before the ANP was induced in the prevention group,and 30 minutes after the ANP was induced in emodin-treated group.The rats in each group were sacrificed at 24 hours after the ANP was induced.The serum levels of renin,angiotensinⅡ,amylase（AMY）,TNF-αand IL-1βwere measured,the pancreatic wet weight/rat＇s body weight of all rats were determined and the pathologic changes of pancreas were analyzed by histopathologic grading and scoring of the severity of pancreas.Results Compared with the control group,there were significant increase in the levels of renin,angiotensinⅡ,amylase,TNF-α,IL-1βand the ratio of pancreatic wet weight/rat＇s body weight in the ANP group;Extensive necrosis,diffuse edema and a large number of inflammatory cell infiltration occurred in pancreatic tissue of the ANP group,and lobular structure of the pancreas were severely damaged.Compared with the ANP group,the serum levels of amylase,TNF-α,IL-1βand the ratio of pancreatic wet weight/rat＇s body weight decreased significantly in the prevention group and emodin-treated group;Pathological lesions alleviated significantly;However,no obvious changes of the serum levels of renin and angiotensinⅡ were found.Conclusion It is suggested that Emodin can decrease significantly the levels of serum AMY,TNF-αand IL-1βin ANP rats,and alleviate the pancreatic pathological damaged conditions,but has no significant effect on the serum levels of renin and angiotensinⅡ.Thus,emodin has an excellent control effcet on the damaged conditions of the ANP.
出处
《中国实验诊断学》
2012年第5期852-855,共4页
Chinese Journal of Laboratory Diagnosis
