摘要
目的研究姜油和6-姜酚对小鼠肝脏UGT、SULT1A1和GST活性及其mRNA表达的影响。方法给小鼠灌服不同剂量的姜油和溶媒对照液,每天2次,连续3天。以分光光度法,检测肝脏中上述代谢酶活性;以RT-PCR法,检测UGT1A1/SULT1A1mRNA在肝脏中的表达。结果姜油(15 mg·kg-1)对小鼠UGT和GST活性有显著的诱导作用(P<0.05或P<0.01);以2-萘酚和4-硝基酚为底物测定SULT1A1活性时,姜油(15,3 mg·kg-1)对SULT1A1均有明显的诱导作用(P<0.05或P<0.01);RT-PCR结果显示,仅有10 mg·kg-1的6-姜酚能够提高UGT1A1/SULT1A1 mRNA的表达。结论临床用药期间,应注意食用姜类产品对Ⅱ相药物代谢酶的影响。
Objective To investigate the effect of ginger oil and 6-gingerol on liver uridine-diphosphate glucuronosyltransferases（UGT）,sulfotransferase 1A1（SULT1A1） and glutathione S-transferase（GST） activities and their mRNA expressions in mice.MethodsThe mice were treated with different dosages of Ginger oil or vehicle respectively two times a day for continuous three days.The activities of UGT,SULT1A1 and GST were detected by spectrophotometry;expression of UGT1A1/SULT1A1 mRNA in liver was estimated by real-time fluorescence quantitative PCR method.ResultsCompared with control group,high-dosage（15 mg·kg^-1） of Ginger oil considerably increased UGT and GST activity（P〈0.05 orP〈0.01）.High and medium-dosage（15,3 mg·kg^-1） of Ginger oil significantly elevated SULT1A1 activity（P〈0.05 or P〈0.01）.RT-PCR results indicated that only 6-Gingerol（10 mg·kg^-1） can induce the expression of UGT1A1/SULT1A1 mRNA in the liver.ConclusionIt is necessary to consider the possible influence of ginger products on the activities of the phaseⅡdrug-metabolizing enzymes.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2012年第5期361-364,共4页
The Chinese Journal of Clinical Pharmacology
关键词
姜油
6-姜酚
葡萄糖醛酸转移酶
硫酸转移酶
谷胱甘肽S-转移酶
Ginger oil
6-gingerol
uridine-diphosphate glucuronosyltransferases
sulfotransferase
glutathione S-transferase
