摘要
目的:建立连翘中槲皮素的HPLC测定方法。方法:采用反相高效液相色谱法,Agilent Eclipse XDB-C18(4.6mm×250mm,5μm)色谱柱;流动相:乙腈-水(32∶68);检测波长:370 nm;柱温:30℃;流速:1.0 mL.min-1;进样量:20μL。结果:不同产地连翘中槲皮素的含量差异显著。结论:此方法可用于检测连翘中槲皮素的含量,为连翘的质量评价提供参考。
Objective： The HPLC method was developed to determine Quercetin in Forsythia suspensa.Method：RP-HPLC was used as follows：Agilent Eclipse XDB-C18（4.6mm×250mm,5 μm）columns;mobile phase：acetonitrile-water solution;the detection wavelength：370nm;column temperature：30 ℃;velocity：1.0 mL·min-1;the injection volume：20 μL.Result：The level of Quercetin in Forsythia suspensa from different commodities is significantly different.Conclusion：The method can be used for the determination of Quercetin in Forsythia suspensa,which provides a scientific basis for quality assessment of Forsythia suspensa.
出处
《中华中医药学刊》
CAS
2012年第5期1143-1144,共2页
Chinese Archives of Traditional Chinese Medicine
关键词
HPLC
连翘
槲皮素
含量测定
HPLC
Forsythia suspensa
Quercetin
determination
