摘要
目的探讨原发陛胆汁陛肝硬化（PBC）患者肝脏雌激素受体（ER-仅）与外周血T淋巴细胞亚群细胞因子和自身抗体的相关性。方法采取横断面研究方法，设未治疗的80例女性PBC患者为PBC组；健康对照组为10例基线匹配的健康女性志愿者；非PBC对照组为20例非自身免疫陛肝病患者，ELISA法检测各组外周细胞因子：白细胞介素6（IL-6）、IL-8、IL_22、肿瘤坏死因子α（TNF-α）、干扰素γ（IFNγ）；自身抗体：M2型抗线粒体抗体（AMA-M2）、抗-Sp00及抗-gp210水平；免疫组织化学法检测PBC组及非PBC对照组肝组织ER-α的表达，指标相关性采用spea彻an相关f生检验，数值资料比较采用秩和检验。结果与健康对照组相比，PBC组和非PBC对照组的细胞因子水平显著升高；而PBC组和非PBC对照组比较，差异无统计学意义。PBC组肝组织ER-cc阳性率为（50．7±8．9）％显著高于非PBC对照组（10．2±2．8）％，z=4．82，P〈0．05，差异有统计学意义。PBC组肝组织ER-α阳性率与血清AMA-M2、抗-SPl00、抗-gp210的阳性率呈正相关，相关系数，分别为0．898、0．819和0．814，P值均〈0．05，差异有统计学意义；ER-α与血清细胞因子IL-22、TNF-α、IFNv的含量也呈正相关，相关系数，分别为0．71、0．89和0．82，P〈0．05，差异有统计学意义。非PBC对照组血清AMA-M2、抗-Sp100、抗-gp210为阴性，肝组织ER-α表达与血清细胞因子水平无相关性（P〉0．05）。结论PBC患者血清细胞因子水平和ER-α阳性率显著升高，与自身抗体阳性率呈正相关，提示PBC患者肝组织高表达ER-α，可能是PBC的发病原因之一。
Objective To investigate the correlation between ER-α in the liver and cytokines of T lymphocytes subsets and serum signatures in PBC patients. Methods The research is performed with cross- sectional study. 80 PBC women patients without treatment were enrolled in PBC group, 10 healthy women as baseline-matched in healthy-control group, and 20 patients with non-autoimmune liver disease in non-PBC control group. The expression of IL-6, IL-8, IL-22, TNF-α, IFNγ AMA-M2, Sp 100 and gp210 were analyzed in Peripheral Blood using ELISA in all groups, and ER-α of patients were performed on tissues from liver biopsies in PBC group and non-PBC control group with immunohistochemistry. Spearman correlation test were performed on the indices to identified the association of all Parameters. numerical data were compared with Wilcoxon rank-sum test. Results Compared with healthy-control group, expression of serum cytokines are significantly higher in PBC and non-PBC groups （P 〈 0.01）, while no significant difference were observed between PBC and non-PBC groups. The positive rate of ER-α in PBC patients liver tissues in PBC group is higher than that in non-PBC group （Z =4.82, P〈 0.01 ）. Expression of ER-α is positively correlated with positive rates of AMA-M2 antibody, Sp 100 and gP210 of tissues of PBC patients （r= 0.898, 0.819, 0.814, P 〈 0.01 ）. ER-α is positive correlated with the expression o f cytokines, among which the coefficient of correlation of IL-22, TNFα, IFNγ is more than 0.7（r=0.71, 0.89, 0.82,P〈0.01）, AMA-M2, Spl00, gp210 is negative in serum of non-PBC control group. No obviously correlations were indicated between the expression of ER-α and cytokines. Conclusion A high level of expression of cytokines in the serum might be one of the factors of etiopathogenisis of PBC.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2012年第5期336-339,共4页
Chinese Journal of Hepatology
基金
国家自然基金（30973824）
关键词
肝硬化
胆汁性
受体
雌激素
细胞因子类
抗体
Liver cirrhosis, biliary
Receptors, estrogen
Cytokines
Antibodies
