摘要
目的了解北京地区动物实验设施动物饮水微生物控制状况。方法根据实验动物无菌动物生活环境国家标准检测方法,对实验动物设施内的动物饮用水样品进行分离培养。扩增分离绿脓杆菌的16SrRNA,并进行测序,绘制发育树。结果在27个参与检测的单位中,未经过处理的饮水无菌生长率41%;经过处理的饮水无菌生长率仅29%。绿脓杆菌检出率30%,主要为RHH13和ZAQ22两个菌株。结论本次检测的实验动物饮水在处理后的效果普遍不甚理想,存在绿脓杆菌的污染,直接影响实验动物质量。
Objective To grasp the microbial control of animal drinking water in laboratory animal facilities of Beijing.Methods According to Method for Examination of Environment and Faeces of GF animal,the drinking water samples of the animal housing were isolated and cultured.16SrRNA of Pseudomonas aeruginosa（PA） isolates that were amplified,and sequenced,then draw phylogenetic tree.Results In the 27 participates of this testing,sterile rate of the pre-treated water is 41%,and the treated water is only 29% in which PA positive is 30%,mainly RHH13 and ZAQ22 two strains.Conclusion The detection of experimental animal＇s drinking water treatment effects is in general less than ideal.PA contamination will directly influence on the quality of laboratory animals.
出处
《实验动物科学》
2012年第1期30-33,共4页
Laboratory Animal Science
关键词
动物饮水
无菌检测
绿脓杆菌
animal＇s drinking water
sterile test
Pseudomonas aeruginosa
