摘要
分析了OsPIL11基因(6个潜在的PIF转录因子之一)的表达模式,结果表明OsPIL11在水稻中的表达具有器官特异性,并且受叶片发育、脱落酸、茉莉酮酸和水杨酸调控。为了进一步分析OsPIL11在植物光信号传导中的作用,构建了OsPIL11基因的过量表达载体,并将其转化至烟草。通过比较转基因和野生型烟草植株幼苗在红光和远红光下的光形态建成特征,发现红光下生长的OsPIL11转基因烟草下胚轴较短、真叶和子叶较大;然而在远红光下,转基因和野生型烟草植株的表型没有差异。这些结果表明,OsPIL11参与红光诱导的幼苗去黄化反应,但是不参与远红光诱导的幼苗去黄化反应。
The expression patterns of OsPILll one of six putative phytochrome-interacting lactors （PIPs）, were analyzed, revealing that expression of OsPILllwas organ-specific and regulated by leaf development, ABA, JA and SA. To further explore the role of OsPILll in plant light signal transduction, plant expression vector of OsPILll was constructed and introduced into tobacco（Nicotiana tabacum）. In continuous red light（R）, OsPILll overexpressed transgenic lines exhibited shorter hypocotyls and larger cotyledons and leaves compared to wild-type seedlings. While in continuous faired light（FR）, either transgenic or wild-type seedlings showed similar phenotypes. These results indica ted that OsPILll was involved in R-induced de etiolation, but not in FR-induced de-etiolation in transgenic tobacco.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2012年第3期255-260,共6页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(30971744
31171182)
河南省科技创新杰出青年计划资助项目(104100510012)
