摘要
考察发酵液中溶解氧对普通生酮基古龙酸菌(Ketogulonicigenium vulgare)和巨大芽胞杆菌(Bacillus magaterium)生长和产酸的影响,优化培养条件以提高维生素C前体-2-酮基-L-古龙酸(2-KLG)的发酵收率。通过比较摇瓶条件下两菌生长和产酸曲线,提出了分三阶段控制通风量策略:即伴生菌巨大芽孢杆菌生长期(0～10h)、产酸菌氧化葡萄糖酸杆菌生长期(10h～25h)和古龙酸大量合成期(25h至发酵结束)。以菌体生长和产酸为衡量指标,对各阶段通风量进行优化,获得三阶段的最适装液量分别为95mL/750mL,95mL/750mL和70mL/750mL。通风量优化后的发酵结果较未优化前,其大菌生长速率、小菌生长速率和古龙酸的产量分别提高了14.7%、10.5%和10.9%。
The aim of this study was to improve the 2-keto-L-gulonic acid（2-KLG） production by Ketogulonicigenium vulgare and Bacillus magaterium.A three-stage ventilation control strategy was developed： the flask content was kept at 95ml/750ml during the first 10h,and maintained at 95ml/750ml for the following 15h,and then decreased to 70ml/750ml till the end of fermentation.With this strategy,K.vulgare growth rate,B.magaterium growth rate and 2-KLG production were increased by 14.7%,10.5% and 10.9%,respectively,compared to those of the control.
出处
《中国酿造》
CAS
2012年第4期144-147,共4页
China Brewing
基金
国家重大新药创制科技重大专项(2011ZX09203-001-14)
关键词
二步发酵
装量调控策略
普通生酮基古龙酸菌
巨大芽胞杆菌
two-step fermentation
flask content control strategy
Ketogulonicigenium vulgare
Bacillus magaterium
