摘要
目的:探讨刺五加总黄酮对大鼠神经细胞脑缺血样损伤模型的保护作用及其机制。方法:采用组织培养法,以原代大鼠大脑皮层神经细胞为材料,制备缺氧损伤、一氧化氮损伤模型,检测刺五加总黄酮的抗缺血作用。结果:形态学检查发现刺五加总黄酮对2种脑缺血样损伤模型中的大鼠神经细胞具有明显保护作用,结晶紫染色也提示刺五加总黄酮可显著提高损伤模型中神经细胞的存活数。结论:刺五加总黄酮对上述大鼠神经细胞缺血损伤均有显著的保护作用。
Objective：To investigate protective effects and mechanism of total flavones of Acanthpanacis Senticosi on ischemia injury models to the rats cortex cells.Methods：Two ischemia models including hypoxia and nitric oxide neurotoxicity were used to assay the anti-ischemic roles of total flavones of Acanthpanacis Senticosi in cultured primary cortex neurons.Results：It was found that total flavones of Acanthpanacis Senticosi possessed obvious protective effects on primary neurons in injury models by means of morphological examination.Crystal violet staining also indicated that total flavones increased number of life neurons in injury models significantly.Conclusion：Total flavones of Acanthpanacis Senticosi protected rats cortex cells from all injury models effectively in vitro.
出处
《牡丹江医学院学报》
2012年第2期3-5,共3页
Journal of Mudanjiang Medical University
基金
牡丹江医学院科研项目(2005-09)
关键词
刺五加总黄酮
神经细胞
脑缺血
Total flavones of Acanthpanacis Senticosi
Cortex neuron
Cerebral ischemia
