摘要
目的探讨通过香烟烟雾暴露将小鼠制备成肺气肿模型,再进一步使其产生系统性炎症形成骨骼肌并发症模型的方法。方法将昆明种系雄性小鼠48只随机平均分为4组:12周正常对照组(A组),24周正常对照组(B组),12周烟雾暴露组(C组),24周烟雾暴露组(D组)。采用单纯长期香烟烟雾被动吸入方式,建立小鼠肺气肿模型。通过肺组织石蜡包埋切片,HE染色观察肺泡结构病理变化,并定量测定平均肺泡数(MAN)和平均肺泡面积量化肺气肿变化程度;进一步测量小鼠体重变化情况,通过该小鼠骨骼肌组织石蜡包埋切片,HE染色观察骨骼肌形态学及结构改变。通过肌间隙产生炎症细胞浸润,肌细胞出现形态结构上的改变,以及局部出现坏死病灶来衡量骨骼肌并发症严重程度。结果观察香烟烟雾暴露小鼠肺组织病理切片发现,肺泡腔扩大、部分肺泡间隔断裂、肺泡腔融合、肺组织肺大泡形成伴周围炎症细胞浸润。A组小鼠平均肺泡面积为(18860±813.2)μm2,B组为(18204±1248.4)μm2,C组为(41787.2±2310.4)μm2,D组为(51577.2±2207.2)μm2。分析发现对照组平均肺泡面积明显低于烟雾暴露组(P<0.01)。烟雾暴露组MAN比正常对照组低(P<0.01)。进一步观察同一例小鼠骨骼肌组织病理切片发现,烟雾暴露组骨骼肌组织中有周围炎症细胞浸润伴随肌细胞形态结构改变,局部性坏死病灶产生,而正常对照组无此改变。结论单纯吸烟法既可以建立肺气肿模型,也可以建立骨骼肌并发症的模型。这为模拟慢性阻塞性肺部疾病(COPD)病人出现系统性炎症,以进行相关科学研究提供了有利条件。
Objective To investigate the modern approach of exposure of cigarette smoke to make mice become emphysema model and further become skeletal complications from systemic inflammation.Methods 48 cases of male Kunming mice were randomly divided into 4 groups：Group A（the control group for 12 weeks）,Group B（the control group for 24 weeks）,Group C（exposure to the cigarette smoke for 12 weeks） and Group D（exposure to the cigarette smoke for 24 weeks）.The method of simple long-term passive inhalation of cigarette smoke was adopted to establish mouse models with emphysema.Sections were embedded in paraffin of lung tissue.HE staining was adopted to observe the pathological changes of alveolus structure.The quantitative measurement of the average number of alveoli（MAN） and the average change in alveolar area to quantify the degree of emphysema were observed.The weight changes of mice were further measured.Sections were embedded in the skeletal muscle tissue of mice with HE staining to observe the morphology and the structural changes of skeletal muscle.The inflammatory cell was infiltrated in muscle space.The morphological changes in the structure of muscle cells as well as the local necrotic lesions were used to measure the change in the severity of skeletal complications.Results After observing the biopsy of lung tissue in the mice exposed to cigarette smoke,it was found that the alveoli expanded,some alveolar septals rupture,alveolar space integrated and the formation of lung bullae with surrounding inflammatory cell infiltration.The average alveolar area of Group A was（18860±813.2） μm2,Group B（1820±1248.4）μm2,Group C（41787.2±2310.4） μm2,Group D（51577.2±2207.2）μm2.Analysis concluded that the average alveolar area of Group A and Group B were significantly lower than those of Group C and Group D（P0.01）.MAN of Group C and Group D was lower than that of Group A and Group B（P0.01）.In the same mice further observation found that in the skeletal muscle of Group B and Group C
出处
《右江医学》
2012年第2期190-193,F0004,共5页
Chinese Youjiang Medical Journal
关键词
烟雾暴露
肺气肿
骨骼肌并发症
小鼠
smoke exposure
emphysema
skeletal complications
mouse
