摘要
目的 观察游离氨基酸在正常人体细胞内外液中的浓度及其在慢性肾功能衰竭（ＣＲＦ）时的代谢变化。方法 对１５例ＣＲＦ患者和１０例健康志愿者的血浆、红细胞内、骨骼肌细胞内的游离氨基酸浓度进行了比较。结果正常人体红细胞内天冬氨酸（Ａｓｐ）、谷氨酸（Ｇｌｕ）和丝氨酸（Ｓｅｒ）浓度较血浆内高（Ｐ＜ ０．０５），而胱氨酸（Ｃｙｓ）、精氨酸（Ａｒｇ）和蛋氨酸（Ｍｅｔ）浓度较血浆内低（ Ｐ＜０．０５），其余大多数游离氨基酸浓度与血浆基本一致；骨骼肌细胞内游离氨基酸浓度普遍较高。ＣＲＦ时血浆中缬氨酸（Ｖａｌ）、亮氨酸（Ｌｅｕ）、异亮氨酸（Ｉｌｅ）、赖氨酸（Ｌｙｓ）、苏氨酸（Ｔｈｒ）、苯丙氨酸（Ｐｈｅ）、Ｓｅｒ、Ｃｙｓ、酪氨酸（Ｔｙｒ）浓度和红细胞内 Ｖａｌ、Ｔｙｒ浓度明显降低（Ｐ＜０．０５），而部分非必需氨基酸浓度升高；骨骼肌细胞内除Ｖａｌ、Ｔｈｒ、Ｌｙｓ浓度降低（Ｐ＜０．０５）外余变化不显著。结论正常人体血浆、红细胞、骨骼肌细胞内游离氨基酸浓度不完全相同；ＣＲＦ时这３种介质中的游离氨基酸代谢均发生改变，主要表现为一些必需氨基酸浓度明显降低，然而降低的幅度３者并不平行，
Objective To investigate the extracellular and intracellular free amino acid concentrations in healthy subjects and its changes in chronic renal failure(Car) .Methods The free amino acid concentrations in the plasma, in the erythrocytes and in the skeletal muscle of 15 patients with CRF were compared with those in ten healthy subjects.Results In healthy subjects, there was little ~difference between the concentrations in erythrocytes and in plasma for most amino acids; but in ~erythrocytes the concentrations of Asp, Glu, Ser were higher than those in plasma (P < 0.05), and the concentrations of Cys,Arg,Met were lower than those in plasma (P<0.05),and the free amino acid concentrations in skeletal muscle were high. In CRF, the concentrations of Val, lie,Leu, Lys,Thr, Phe,Ser, Cys, Tyr in plasma, Val, Thr in erythrocytes and Val, Thr, Lys in skeletal muscle were lower than those in healthy subjects(P < 0.05), but the concentrations of several non-essential amino acids in erythrocytes and in plasma ran high(P < 0.05). Conclusions The free amino acid concentrations in plasma, in erythrocytes and in skeletal muscle are different in healthy subjects and disturbed in patients with CRF. In CRF, concentrations of some essential amino acids decrease in above the, but the lowering ranges are not parallel among them.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2000年第1期31-33,共3页
Chinese Journal of Nephrology
关键词
慢性
肾功能衰竭
细胞内液
细胞外液
氨基酸
Chronic renal failure
Intracellular fluid
Extracellular fluid
Free amino acid
