摘要
目的:采用正交试验设计方法进行肺炎链球菌5型发酵工艺的研究。方法:根据正交试验设计表L9(34)设计的试验条件组合进行了9次肺炎链球菌5型的发酵,采用70升发酵罐进行发酵工艺的摸索,提取了肺炎链球菌5型荚膜多糖粗糖。结果:最佳的发酵培养条件组合为温度37℃、葡萄糖20克/升、大豆胨15克/升、pH值7.3,最佳的纯化条件组合为冷酚抽提三次、沉核酸乙醇浓度23%、超滤膜孔径50kD、最终沉糖乙醇浓度60%,在此筛选得到的最佳条件下,连续进行了5个批次肺炎链球菌5型的发酵与荚膜多糖提取,荚膜多糖粗糖的平均收率为808.6mg/L,相对标准偏差为3.84%。结论:上述发酵培养条件组合适合用于肺炎多糖疫苗的研究和生产。
Objective：To investigate a good fermentation process of Streptococcus pneumoniae serotype 5 and capsular polysaccharide purification process of Streptococcus pneumoniae serotype 5.Methods： Streptococcus pneumoniae serotype 5 was cultured in 70 L fermenter with orthogonal experimental design,and purifying capsular polysaccharide.Results： The main quality indexes of the capsular polysaccharide of Streptococcus pneumoniae serotype 5 met the requirements of Europe Pharmacopeia 5.0.Conclusion： A successful process for large-scale production of Streptococcus pneumoniae serotype 5 capsular polysaccharide was established,and this procedure is suitable for pneumococcal vaccine production.
出处
《现代生物医学进展》
CAS
2012年第9期1660-1664,共5页
Progress in Modern Biomedicine
关键词
肺炎链球菌
发酵
正交试验设计
荚膜多糖
纯化
Streptococcus pneumoniae； Fermentation； Orthogonal experimental design； Capsular polysaccharide； Purification；
