摘要
目的观察药物性急性间质性肾炎（D-AIN）肾组织浸润炎性细胞的特征及意义。方法将重复肾活检的D-AIN患者12例分为肾功能完全恢复组（A组）和未完全恢复组（B组），每组6例。应用免疫组化法观察T淋巴细胞[CD3（＋）]、单核巨噬细胞[CD68（＋）]及B淋巴细胞[CD20（＋）]的浸润、肾间质微血管密度以及细胞外基质的沉积。结果①2组性别、基础肾功能和病理损害程度等无明显差异；B组第2次肾活检时出现肾间质纤维化。②第1次肾活检时，2组均表现为显著的炎症细胞浸润。A组第2次肾活检时各种炎症细胞均明显减少，而B组[CD68（＋）]细胞未受抑制，成为主要的浸润细胞，伴有胶原沉积明显增加。结论持续单核巨噬细胞浸润可能是D-AIN患者病变迁延的重要机制。
Objective To observe the characteristics of the inflammatory cells in drug-induced acute interstitial nephritis （D-AIN）. Methods Twelve cases of D-AIN subject to renal re-biopsy were divided into recovered and non-recovered groups. Immunohistochernical SP method was applied to detect the infiltration of T lymphocytes [CD 3 （ ＋ ）], monocytes/macrophages[CD 68 （ ＋ ）] and B lymphocytes [CD 20 （ ＋ ）], the density of peritubular capillaries [PTC, CD 34（ ＋ ）], and the expression of a-SMA, collagen III and IV in the renal biopsy specimens. Results The two groups presented comparative clinicopathological features, as well as the degrees of inflammatory cells infiltration. After treatment for 1-4 months, patients in the recovered group showed significant decrease of inflammatory infiltration in the re-biopsy kidneys, while those in the non-recovered group presented persistent monocytes/macrophages infiltration and significant interstitial fibro- sis. Conclusions Monocytes/macrophages might play very important roles in the transition from acute in- flammation into chronic fibrosis in D-AIN.
出处
《临床肾脏病杂志》
2012年第4期155-158,共4页
Journal Of Clinical Nephrology
基金
国家自然科学基金
