摘要
提出了高效液相色谱法测定消毒湿巾中苯扎氯铵含量的方法。样品经流动相超声提取,以Eclipse XDB-C18色谱柱(150 mm×4.6 mm,5μm)为分离柱,以乙腈-70 mmol.L-1乙酸铵(含1%三乙胺,冰乙酸调pH至5.0)按体积比70比30混合液为流动相,用二极管阵列检测器于波长262 nm处测定。苯扎氯铵的质量浓度在100～500 mg.L-1范围内与其峰面积呈线性关系,检出限(3S/N)为10.2mg.L-1。应用此法测定消毒湿巾中苯扎氯铵,回收率在95.3%～97.8%之间,相对标准偏差(n=5)在2.5%～4.0%之间。
A method for determination of benzalkonium chloride in disinfectant wet wipes by HPLC was proposed. The sample was extracted ultrasonically with the mobile phase, and then separated on Eclipse XDB-C18 （150 min×4. 6 mm, 5 μm） column, using a mixture of acetonitrile and 70 mmol · L^-1 ammonium acetate solution （containing 1% by vol. of triethylamine, adjusted with acetic acid to pH 5.0） in the ratio of 70 to 30 as mobile phase. Photodiode array detections were made at 262 nm. Linear relationships between values of peak areas and mass concentration of benzalkonium chloride was obtained in the range of 100--500 mg· L^-1 ,with limit of detection （3S/N） of 10. 2 mg · L^-1. The proposed method was used in the analysis of sample of disinfectant wet wipes, values of recovery found by standard addition method were in range of 95.3%-97.8% with RSD＇s （n=5） in the range of 2.5 %- 4.0%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2012年第4期400-401,共2页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
