摘要
为获得与草莓炭疽病密切相关的分子标记,需构建高密度与抗病相关的遗传连锁图,本研究以易感草莓炭疽病品种宝交早生(Hokowase)与高抗草莓炭疽病品种甜查理(Sweet Charlie)杂交的210个F2代群体材料为作图群体,构建了包含34个AFLP标记和109个SSR标记的分子遗传图谱,并对抗草莓炭疽病相关因素进行了QTL分析。该图谱共包括7个连锁群和133个遗传标记,平均每个连锁群有19个遗传标记。遗传图谱总覆盖长度为451.8cM,标记间平均距离为3.4cM。经复合区间作图法分析得到3个与草莓炭疽病抗性相关基因座(QTL),其中2个与Colletotrichum acutatum抗性相关,分布在LG3和LG5连锁群上,可解释表型变异的31.6%;1个与Colletotrichum gloeosporioides抗性相关,分布在LG6连锁群上,可解释表型变异的68.4%。
Strawberry anthracnose, caused by the Colletotrichum species, is the major production constraint in rangtze River valley with humid summer and autumn. Normally, control of the disease relies on frequent and regular fungicide applications. Modern strawberry breeding programs including the use of molecular markers, makes it possible to combine several different resistance genes. A genetic map is necessary for the reliable detection, mapping and estimation of gene effects of anthracnose resistance of strawberry genome. In this study, simple sequence repeats （SSRs） and amplified fragment length polymorphism （AFLP） were used to assay an F2 population from a cross between Hokowase （ Fragaria x ananassa Duchase） and Sweet Charlie （Fragaria x ananassa Duehase）. 210 F2 plants were used for map construction using 109 SSRs and 34 AFLPs, including 6 strawberry probes which correspond to the known genes. This map consisted of 143 markers distributed in seven linkage groups, covering 451.8cM with an average distance of 3.4cM between two adjacent markers and only three gaps of 〉5cM were found. The linkage map was located on 3 linkage groups, i.e. LG3, LG5 and LG6. Based on composite interval mapping, two QTLs were identified for c. acutatum resistance i. e. on LG3, LG5, explaining 31.6% of the resistance disease variance. One QTLs was identified for C. gloeosporioidesresistance on LG6, explaining 68.4% of the resistance disease variance.
出处
《核农学报》
CAS
CSCD
北大核心
2012年第2期250-256,269,共8页
Journal of Nuclear Agricultural Sciences
基金
上海市科技兴农重点攻关项目(沪农科攻字(2008)第1-4号)
上海市科委自然科学基金项目(10ZR1426700)
上海市科委青年科技启明星计划(09QA1405300)
2010年度农业部公益性行业(农业)科研专项(No.201003064-5)
上海市农委科技兴农重大项目(沪农科重字(2010)第4-1号)
