摘要
目的探讨应用高效液相色谱串联质谱法(HPLC-MS/MS)测定血浆中西酞普兰药物浓度的方法。方法设立色谱柱为Agilent-ZORBAX-C8柱;流动相:乙腈-0.1%甲酸水溶液(60:40);流速:0.5 ml/min;柱温:20℃;质谱条件为电喷雾电离源(ESI),以多反应监测(MRM)方式进行检测,用于定量分析的反应离子为m/z 325.1 262.1(西酞普兰)和m/z256.2 67.1(苯海拉明,内标)。结果西酞普兰在0.5～100 ng/ml范围内线性关系良好(r=0.9979);西酞普兰的日内、日间精密度RSD均<10%。结论该方法分析速度快,专属性强,灵敏度高,可作为西酞普兰的血浆药物浓度检测方法。
Objective To establish an HPLC-MS/MS method for determination of the content of citalopram in human plasma.Methods The chromatographic column was Agilent-ZORBAX-C8（4.6×150 mm,5 μm）,from Agilent Company,The mobile phase was methyl cyanides-0.1%formic acid（60∶ 40）.The flow rate was 0.5 ml/min and m/z 256.2167.1.The column temperature was 20 ℃.Electrospray ionization（ESI） source was applied and multi-reaction monitoring（MRM） mode with the transitions of m/z 325.1262.1（for citalopram） and m/z 256.2167.1（for diphenhydramine internal standard）was used.Results The assay linearity of citalopram was confirmed over the range 0.5-100 ng/ml（r=0.9979）.The intra-and inter-day precision（RSD） was less than 10%.Conclusions The method is proved to be quick,specific,sensitive and accurate,which can be used for the determination of citalopram in plasma.
出处
《武警医学》
CAS
2012年第4期323-326,共4页
Medical Journal of the Chinese People's Armed Police Force
