摘要
用紫外-可见吸收光谱、X射线衍射、荧光光谱和衰减全反射傅里叶变换红外(ATR-FTIR)光谱等方法,研究了烟酰胺腺嘌呤二核苷酸(NAD+)在纳米α-Al2O3粒子上的吸附行为。实验结果显示,NAD+的吸附量受pH值和离子强度影响较大,说明NAD+主要通过静电作用吸附在纳米α-Al2O3粒子上。采用ATR-FTIR光谱分析了不同pH值溶液中及被吸附的NAD+,发现吸附后的NAD+与溶液中NAD+的ATR-FTIR光谱相似,但磷酸根的吸收峰向高波数位移,说明磷酸根参与了表面静电作用。吸附过程符合Langmuir和Freundlich等温式。荧光实验结果显示,随着吸附剂α-Al2O3用量的变化,NAD+构象也发生变化。
The adsorption behavior of nicotinamide adenine dinucleotide（NAD＋） on α-Al2O3 nanoparticles was studied using ultraviolet-visible（UV-Vis） absorption spectroscopy,X-ray diffraction（XRD）,fluorescence and attenuated total reflectance-Fourier transform infrared（ATR-FTIR） spectroscopy.It was found that the amount of adsorbed NAD＋ exhibited a strong dependence on pH and ionic strength of the solution,which suggested that the adsorption of NAD＋ at α-Al2O3 nanoparticles was caused predominantly by the electrostatic adsorption.ATR-FTIR spectra of adsorbed NAD＋ were similar to those of NAD＋ in solution.However,the position of peaks for phosphate moving to slightly higher wavenumbers suggested that phosphate participated into the electrostatic adsorption.The adsorption isotherm could be fitted with both Langmuir and Freundlich isotherms.The fluorescence spectral features showed that the conformation of NAD＋ changed with the change of the amount of α-Al2O3 nanoparticles.
出处
《应用化学》
CAS
CSCD
北大核心
2012年第5期551-556,共6页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(20875047,20873065)重点基金(20833006)
江苏高校优势学科建设工程资助项目
关键词
吸附
烟酰胺腺嘌呤二核苷酸
静电作用
构象
adsorption
nicotinamide adenine dinucleotide
electrostatic interaction
conformation
