摘要
目的 :观察海马区星形胶质细胞的活化状态与缺血耐受性的关系。方法 :钳夹沙土鼠的双侧颈总动脉制造脑缺血模型 ,应用尼氏染色及免疫荧光法染色 ,观察海马锥体细胞迟发性神经元坏死及抗胶质纤维酸性蛋白 (GFAP)染色的改变。结果 :脑缺血组 ,CA1区锥体细胞几乎全部丧失 ,只见很少量的细胞残存 ,零星散在分布 ;预缺血组 ,CA1区锥体细胞少部分丧失 ,大部分幸存。对照组有少量微弱的GFAP染色阳性细胞 ;脑缺血组有些GFAP染色阳性细胞 ,多为弱阳性 ;预缺血组GFAP染色反应明显增多、增粗 ,且染色深染。结论 :星形胶质细胞形态、机能的改变在缺血性脑损伤中起重要作用 。
AIM:To observe the relation between astroglial activation state and ischemic tolerance in the gerbil hippocampus. METHOD: Bilateral occlusion of common carotid arteries and immunofluorescent methods in the gerbil hippocampal tissue. Slices were used. The morphological changes of the neurons were observed by light microscopy.RESULTS:Pretreatment with 2 minute bilateral carotid artery occlusion produced protective effects of CA 1 neurons. Glial fibrillary acidic protein (GFAP) staining showed weak positive cells in control group. Most of GFAP positive cells showed weak and middle positive cells after recirculation 1d and 2d following ischemic 3.5 min and preconditioning of the brain with sublethal ischemia respectively.CONCLUSION:Astroglial played an important role in cerebral ischemia. It is possible that state of astroglial activation related to neuronal survival in ischemic tolerance.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第2期160-162,共3页
Chinese Journal of Pathophysiology
基金
山东省自然科学基金资助! (Y98C190 48)