摘要
为了解环太湖水域微囊藻毒素(MC-LR)在螺蛳体内的积累情况,实验模拟螺蛳生长环境,对养殖水人为添加毒素,按毒素质量浓度分为低毒组(8μg/L)和高毒组(80μg/L),实验为期50d,每隔10d进行取样,采用液-质联用方法对螺蛳可食组织、不可食组织的MC-LR含量进行检测。结果显示:各组织均有藻毒素积累,可食组织中MC-LR积累量缓慢持续上升,不可食组织中MC-LR积累呈波浪形上升;不可食组织(MC-LR含量最大值为14.94μg/kg)对MC-LR的积累能力明显大于可食组织(MC-LR含量最大值为2.49μg/kg);高毒组螺蛳体内MC-LR的最大积累量是低毒组的6倍。根据WHO藻毒素的限量标准(1μg/L)推算出螺蛳可食组织MC-LR积累量属安全范围,但仍可能存在食品安全风险。
In order to understand the accumulation of water bloom microcystin in herbivorous aquatic snails, the standard toxin MC-LR was added in snail aquaculture water at the concentrations of 8μg/L and 80 μg/L, resulting in low and high toxicity groups. The feeding time lasted 50 days and liquid chromatography-mass spectrometry (LC-MS) was used to determine the amount of the toxin in different organs from the snails every other 10 days. The results showed that all investigated tissues had accumulation of MC-LR. The accumulation capacity of the toxin in inedible tissues ( 14.94 μg/kg at the maximum level) of snails was much more than that in edible tissues (2.49 μg/kg at the maximum level). In addition, the maximum accumulation in the high toxicity group presented a 6-fold increase when compared with the low toxicity group. The microcystic toxin accumulation in edible tissues from snails was in the safe range according to the WHO limit (1μ g/L) standard, but risk may still exist at the same time.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第7期280-283,共4页
Food Science
基金
无锡市科技局指导性计划项目(CSZ00958)
江苏省卫生厅科研基金项目(HD200865)