摘要
目的:研究IL-13基因多态性位点rs20541和rs1800925单核苷酸多态性与儿童支气管哮喘发病的关联性。方法:应用聚合酶链反应(PCR)、等位基因特异性PCR(AS-PCR)和直接测序方法检测105例儿童哮喘患者和112名正常儿童的IL-13基因上rs20541和rs1800925单核苷酸多态性分布特点。结果:第4外显子中的+2044G→A(rs20541)哮喘组与正常对照组呈现明显差异(P<0.05)。而-1112C→T(rs1800925)位点两组间差异无统计学意义(P>0.05)。结论:+2044G→A(rs20541)与哮喘患儿的发病具有强烈相关性。-1112C→T无明显相关性。
Objective： To evaluate the association between the IL-13 gene rs20541 and rs1800925 single nucleotide polymorphisms and asthma in children. Methods： Polymerase chain reaction （PCR）, allele-specific PCR （AS-PCR）,and direct sequencing were used to detect the IL-13 gene rs20541 and rs1800925 single nucleotide polymorphism distribution in 105 cases of pediatric asthma and 112 normal children. Results： Of the ＋2044 G→ A （rs20541） site in the exon 4,the patient and normal control groups showed significant differences（P〈0.05）. No significant difference in the-lll2C→T （rs1800925） site was noted between the 2 groups. Conclusion： The rs20541 site has a strong correlation to the occurrence of asthma, while no significant correlation was seen in rs1800925.
出处
《浙江中西医结合杂志》
2012年第4期254-255,305,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
