摘要
目的研究分枝杆菌噬菌体D29的生物学特性,为D29抗耐药结核治疗奠定基础。方法观察噬菌体电镜结构和噬菌斑形态;测定D29最佳感染复数(MOI);一步生长实验;检测pH值对D29活力的影响;斑点法测定裂解谱;中和实验检测抗原性结果D29噬菌斑圆形透明,边界清楚;D29尾长129 nm,最佳MOI为10^(-4);D29感染宿主菌的潜伏期约为50 min,裂解量为10;pH值对D29存活率影响大,酸性环境不影响D29裂解能力;D29能裂解分枝杆菌临床耐药株;D29 K值为1069.50。结论 D29属于长尾噬菌体科(siphoviridae),裂解谱广,抗原性较高,具有抗耐药结核潜力。
Objective To characterize Bacteriophage D29 of Mycobacterium and providethe base for future exploration of phages in control of drug-resistant tuberculosis. Methods We observed morphological property of the plaques and uhrastructure of D29. We examined D29＇s optimal multiplicity of infection and its single step growth curve. And also we inspected how D29＇s survived was affected by pH value. The host range of phage D29 was determined by Spot Test. The reaction constants between antiserum and D29 was identified by neutralization test. Results The plaques of D29 are transparent and their sizes are about 5 mm in diameter. D29 has a long tail about 129 nm in length. Host bacteria infected by 0. 000 1 MOI produced the highest phage offsprings. The single step growth test showed that, phage D29 had a latent period of 50 minutes and a burst size of 10. pH value had large influence on the survival rate of phage D29, but pH5 did not affect the activity of D29 Lysis of bacteria. The susceptibility to phage D29 was also investigated with other clinical strains of Mycobacterium except H37Rv. Rate constoat of reaction between antiserum and D29 is 1069.50. Conclusion Phage D29 is a suitable broad host range and high antigenicity siphoviridae phage which could be used in phage therapy.
出处
《世界科技研究与发展》
CSCD
2012年第2期314-317,共4页
World Sci-Tech R&D
基金
国家"十一五"科技重大专项(2008ZX10003-016)资助
关键词
分枝杆菌
噬菌体
生物学
耐药结核
mycabacterium
bacteriophage
biology
drug-resistant tuberculosis
