摘要
目的了解磷酸化cofilin(P-cofilin)在癫痫脑组织中的表达情况,以探讨cofilin磷酸化形式在癫痫发生、发展申的作用。方法应用蛋白免疫印迹和免疫组化技术检测氯化锂-匹罗卡品大鼠癫痫模型癫痫大发作后各时间点脑组织中P-cofilin的表达水平;并在难治性癫痫患者脑组织中检测P-cofilin的表达情况。结果在氯化锂-匹罗卡品大鼠癫痫模型的24小时、7天和14天P-cofilin表达水平相比正常对照明显下降(P<0.05),而在72小时和60天中P-cofilin表达水平明显升高(P<0.05);而在难治性癫痫患者脑组织中P-cofilin表达水平比非癫痫患者高(P<0.05)。结论 P-cofilin在氯化锂-匹罗卡品大鼠癫痫模型和难治性癫痫患者脑组织中有明显异常的表达,可能通过易化、稳定异常兴奋性环路而参与癫痫或难治性癫痫的发生、发展。
Objective To investigate P-cofilin exoression in epilepsy in order to exploer the possible roles of P-cofilin in the pathogenesis of epilepsy. Methods P-cofilin expression was detected in the rat epileptic model of Lithium-pilocarpine and in patients with drug-refractory epilepsy using western blot and immunohistochemistry. Results Comparing with control rats, P-cofilin expression was down-regulated at time points of 24 h,7 d and 14 d in the rat epileptic model of Lithium-piloearpine （ P 〈 0. 05 ）, but was up-regulated at time points of 72 h and 60 d（ P 〈0. 05 ）. In patients with drug-refractory epilepsy P-cofilin expression was higher than the control group （P 〈 0. 05 ）. Conclusion The abnormal expression of P-cofilin in the rat epileptic model of Lithium-pilocapine and in patients with drug-refractory epilepsy may facilitate the formation of the excitability of abnormal loop and epilepsy.
出处
《世界科技研究与发展》
CSCD
2012年第2期311-313,348,共4页
World Sci-Tech R&D
基金
重庆市自然科学基金计划项日任务书(CSCT 2010BB5106)
重庆市卫生局医学科研重点项目(2010-1-36)资助
