摘要
用TTC法测定油茶花粉散粉后的活力变化及寿命,用琼脂培养基萌发法测定了花粉萌发率,用联苯胺-过氧化氢法测定了柱头可授性。结果表明:油茶花粉活力在散粉8 h呈上升趋势,即散粉第1～2天保持较高活力,随后活力开始下降,花粉寿命为4～10 d,最佳传粉期为开花后第1～5天;油茶花粉萌发率是体现花粉活力的一个指标,结果略低于TTC法测定的花粉活力值,它是对TTC法测定花粉活力的补充和验证;油茶柱头可授性持续3～6 d,花苞在将开而未开时已具备一定可授性,开花第1～5天柱头可授性呈上升趋势,第5～10天可授性减弱至基本消失;与柱头可授性相比,花粉寿命相对长些,试验结果与所观测的单花开放寿命较相一致。
TTC method was utilized to determine the vigor change and life-span of the Camellia oleifera pollen after anther dehiscence;with agar medium germination method,the pollen germination rate was determined;and with benzidine-hydrogen peroxide method,the stigma receptivity was measured.The results show that Camellia oleifera pollen vigor showed a rising trend in 8 hours after anther dehiscence,within 1～2 days the pollen maintained at a high vigor level,then gradually declined,the life-span of Camellia oleifer pollen was around 4～10 days or so,and the best pollination period was 1 to 5 days after flowering;The rate of Camellia oleifer pollen germination is an indicator of pollen vigor,the determining value was slightly lower than the result of the TTC method,which is a kind of supplement and verification to TTC method in determining the pollen vigor;The stigma receptivity maintained for 3～6 days or so,but the best pollination period was about 5 days,among open in the bud and the un-blossomed the flowers already have a certain receptivity,flowering the first 1～5 days the stigma receptivity was rising,5～10 days started to reduce until to basically disappeared;Compared with the period of stigma receptivity to pollen,life-span was relatively longer,the test results and the observation of a single flower opening life was consistent.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2012年第3期17-22,共6页
Journal of Central South University of Forestry & Technology
基金
湖南省科技厅科技计划重点项目"油茶优良种质资源遗传背景数据库建设与规模快繁技术研究"(2010FJ2010)
中央财政林业科技推广示范跨区域重点推广示范项目"油茶杂交新品种及丰产栽培技术集成示范"([2011]TK074号)
林业公益性行业科研专项"油茶两系杂交种子园营建及高产培育技术研究"(200804044)
关键词
油茶
散粉
花粉活力
萌发率
柱头可授性
Camellia oleifera
anther dehiscence
pollen vigor
pollen germination rate
chapiter receptivity
