摘要
从活性污泥中筛选出的C-2苏云金芽孢杆菌,能耐受250mg/L的六价铬,并具有较好的还原能力。研究表明木糖、果糖、玉米饼粉、苹果酸、琥珀酸、柠檬酸及Cu2+、Fe2+、Ca2+离子对C-2菌的还原有积极作用,菌体的接种量影响还原的速率。C-2菌还原的最适温度为37℃,最适pH为9.0;六价铬还原酶的最适pH为7.0、温度为37℃,Co2+、Cu2+、Fe2+、DTT、NADH对酶的还原有积极影响。
The Bacillus thuringiensis C-2 strain was isolated from activated sludge and it can tolerant 250mg/L Cr6＋ and have better reducing capacity.The results showed that xylose,fructose,corn cake powder,malic acid,succinic acid,citric acid and ions Cu2＋、Fe2＋、Ca2＋ had an active role for reduction and the inoculum size had influence for reduction.The conditions of 37℃ and pH9.0 were good for C-2 strain to reduce the Cr6＋.The optimum pH and temperature of chromium reductase was pH7.0 and 37℃.The Co2＋,Cu2＋,Fe2＋,DTT,NADH effectively promoted the reduction.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2012年第4期53-59,共7页
China Biotechnology
基金
河南省超级产粮大省奖励资金资助项目(2001-196-31)
关键词
C-2菌
六价铬
还原酶
C-2 strain Hexavalent chromium Reductase
