摘要
目的:建立不同产地单芽狗脊蕨HPLC指纹图谱测定方法。方法:采用HPLC测定了9个不同产地单芽狗脊蕨样品。色谱条件为DiamonsilTMC18色谱柱(4.6 mm×200 mm,5μm);流动相A相为乙腈,B相为水(梯度洗脱0～60 min 22%A→100%A);检测波长265 nm;流速1.0 mL·min-1;柱温25℃。结果:建立了不同产地单芽狗脊蕨的HPLC指纹图谱,并进行了相似度比较,进一步对其中的山柰素-3-O-α-L-鼠李糖基-7-O-α-L-鼠李糖苷、山柰素-3-O-α-L-(4-O-乙酰基)鼠李糖基-7-O-α-L-鼠李糖苷进行了归属。结论:该法操作简便、重复性好,可用于单芽狗脊蕨质量控制。
Objective： To establish the fingerprints of Woodwardiae unigemmmata from different habitats by HPLC.Method： HPLC was applied to detect W.unigemmmata samples from 9 different habitats with a DiamonsilTMC18 column by acetonitrile A-water B as mobile phase（gradient elute： 0-60 min,22%-100%A）.The flow rate was 1.0 mL·min-1.The column temperature was at 25 ℃ and the UV detection wavelength was set at 265 nm.Result： The HPLC fingerprints of W.unigemmmata from different habitats were established and the correlation coefficients of them were calculated.Furthermore,two peaks in HPLC chromatogram were identified as kaempferol-3-O-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside,kaempferol-3-O-α-L-（4-O-acetyl）-rhamnopyranoside-7-O-α-L-rhamnopyran-oside respectively.Conclusion： The method is simple,reproducible and can be used for quality control of W.unigemmmata.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第9期151-153,共3页
Chinese Journal of Experimental Traditional Medical Formulae
