摘要
目的比较负载血管内皮生长因子(vascular endothelial growth factor,VEGF)DNA质粒胶原-磺化羧甲基壳聚糖真皮支架及胶原-磺化羧甲基壳聚糖真皮支架在修复III度烧伤创面中对创面收缩与细胞凋亡的影响。方法将两种不同真皮支架各移植于6头猪(共12头猪)III度烧伤清创后创面,对植入支架1、2、3周后的创面及支架植入2周加植表皮2周的创面修复情况进行观察。同时,通过采用免疫组织化学方法,对不同时间的创面中表达α-SMA的成熟血管和肌成纤维细胞以及细胞凋亡发生情况进行检测和观察。以不植入支架的烧伤创面作为实验对照。结果①植入不同真皮支架的创面与无支架植入的肉芽创面不同,负载VEGF DNA质粒真皮支架修复创面效果好于无质粒负载真皮支架。②在两种不同支架植入后1～3周创面α-SMA表达阳性的血管数持续增加,支架植入2周加植表皮2周血管数比3周创面明显减少,不同时间点α-SMA表达阳性的血管以负载VEGF DNA质粒的胶原-磺化羧甲基壳聚糖人工真皮支架植入创面最多,无支架植入创面最少;③α-SMA表达阳性的肌成纤维细胞以负载VEGF DNA质粒的胶原-磺化羧甲基壳聚糖人工真皮支架植入创面最少,无支架植入创面最多,负载VEGF DNA质粒胶原-磺化羧甲基壳聚糖真皮支架及胶原-磺化羧甲基壳聚糖真皮支架创面中表达高峰为2周,无支架创面表达高峰为3周。④不同支架及无支架创面植入后2～4周,创面内细胞凋亡持续大幅增多,且无支架创面中发生细胞凋亡最少。结论负载VEGF DNA质粒的胶原-磺化羧甲基壳聚糖人工真皮支架在烧伤创面愈合过程中可促进血管化,通过抑制创面收缩和促进细胞凋亡减少瘢痕的产生。
Objective To analyze effects of collagen-sulfonated earboxymethyl chitosan porous scaffolds containing vascular endothelial growth factor （VEGF） DNAs on celluar apoptosis and wound contraction during burn wound repair. Methods Scaffold transplantations were performed for grade III burn wounds in 12 Bama miniature pigs following a 3-week wound repair observation. Epidermis of pigs were grafted at week 2 after scaffold transplantations. The treatment group was transplanted by eollagen-sulfonated carboxymethyl chitosan porous scaffolds containing vascular endothelial growth factor （VEC, F） DNAs and the negtivecontrol group was only with the same kind of scaffold, in addition to the blank control group without any saefflod. Immunohistochemical staining was used to analyze ot-SMA expression and apoptosis of myo-fibroblasts and mature vessels at different times. Results （1） The conditions of granulation wounds transplanted by different scaffolds were different. The effect of wound repair was better in the treatment group, compared with the negative control and blank control groups. （2）Vessels expressing α-SMA in wounds increased continuously in 3 weeks after different transplantations, especially in the treatment group. However, treatment with epidermis graft after scaffold transplantations decreaseds the number of α-SMA-expressing vessels. （3）The number of myo-fibroblasts expressing α-SMA in wounds was smallest at the treatment groups, compared to other two groups. （4） Celluar apoptosis was found in each group and the blank group had the lowest intensity of apoptosis. Conclusion The collagen-sulfonated carboxymethyl chitosan porous scaffold containing VEGF DNAs can effectively induce angiogenesis, and inhibit proliferation of scar through reducing wound contraction and inducing apoptosis.
出处
《健康研究》
CAS
2012年第2期89-93,F0002,共6页
Health Research
基金
浙江省大学生科技创新活动计划(0813XXZ08)
关键词
人工真皮支架
血管化
创面修复
Ⅲ度烧伤
创面收缩
细胞凋亡
Artificial dermal scaffold
angiogenesis
wound repair, full-thickness burn
wound contraction
apoptosis
