摘要
目的:建立口腔黏膜白斑(OLK)基因诊断方法,为临床OLK的早期诊断提供研究基础。方法:采用RT-PCR技术检测OLK组织和非OLK组织的肿瘤相关基因(NF-1、ACP-2、BCL-2、CLK-3、FKBP-8、SOCS-3、XRCC-1、CTNNB-1、GDF-15)表达水平,使用SPSS 11.5软件建立数据库,采用Fisher判别分析建立口腔黏膜白斑基因诊断方法,采用Cross-validated(a)法对本判别方法进行评价。结果:本研究建立了口腔黏膜组织是否为OLK组织的判别公式为:Y=-27.503+0.094XGDF-15-0.122XNF-1+0.368XSOCS-3,确定了判别界值Yc=-1.186,即大于-1.186为非OLK组织,小于-1.186为OLK组织。总判别符合率为、交互判别符合率、灵敏度、特异度均为100%。结论:本研究为临床OLK的基因诊断提供了简便易行的操作方法。
Objective：To establish the gene diagnostic method of oral leukoplakia（OLK） and to make research base for clinical early diagnosis of OLK.Methods：OLK tissue and non-OLK tissue were detected by RT-PCR to obtain tumor-related genes（NF-1,ACP-2,BCL-2,CLK-3,FKBP-8,SOCS-3,XRCC-1,CTNNB-1,GDF-15） expression levels.A database was established using SPSS 11.5 software,and a discriminant equation was inferred by Fisher discriminant analysis.Cross-validated（a） method was used to evaluate the discriminant method.Results：In this study,a discriminant equation was established to discriminate OLK tissues.The formula was Y=-27.503 ＋0.094 X（GDF-15）-0.122X（NF-1） ＋0.368 X（SOCS-3）,and the discriminant cutoff Yc=-1.186,that was：greater than-1.186 for non-OLK tissue,less than-1.186 for the OLK tissue.The total identification rate was 100%,the interaction determine rate was 100.00%,the sensitivity was 100%,and the specificity was 100%.Conclusion：In this study,a clinical gene diagnosis method was provided for OLK.
出处
《中国卫生检验杂志》
CAS
北大核心
2012年第4期691-694,共4页
Chinese Journal of Health Laboratory Technology
基金
太原市技术创新计划项目(0701026)
山西省科技攻关项目(09122058)
山西省科技攻关项目(20090322028-1)
