摘要
采用PEG ( 60 0 0 )根际渗透胁迫处理 ,测定了水分胁迫下马尾松、火炬松、水杉幼苗硝酸还原酶 (NR)和超氧化物歧化酶 (SOD)活性变化。结果表明 ,在水分胁迫下各苗木NR和SOD活力都呈下降趋势。NR和SOD活性以及RWC两两间均呈明显的线性正相关。从 3个树种抗旱性与NR和SOD活性下降程度来看 ,抗旱性较强的马尾松NR和SOD活性下降幅度均最小 ;抗旱性较差的水杉NR活性下降幅度最大 ,SOD居第 2位 ,抗旱性居中的火炬松NR活性下降幅度居第 2位 ,而SOD居第 1位。表明 3个树种抗旱性与胁迫下NR活性呈明显正相关 ,而与SOD活性没有明显的相关性。NR活性可以作为这 3个树种间抗旱能力评定的有效生化指标 ,SOD活性可以作为参考指标。表 2参 1
The activities of nitrate reductase (NR) and superoxide dismutase (SOD) were studied in seedlings of Pinus massoniana,Pinus taeda and Metasequoia glyptostrobides under osmotic stress by PEG(6000).The results showed that the activities of NR and SOD were all decreased in three seedlings under stress.The order of NR activity under stress was Pinus massoniana>Pinus taeda>Metasequoia glyptostroboides.The order of SOD activity was Pinus massoniana>Metasequoia glyptostroboides>Pinus taeda.Because the droughtresistance ablilty was Pinus massoniana>Pinus taeda>Metasequoia glyptostroboides(based on the former studies),there was a positive correlation between the activity of NR and drought resistance in these seedlings under water stress,but there was no significant correlation between the SOD activity and drought resistance.The conclusion obtained from this study reflected that the activity of NR could be used as an effective biochemical index for the criterion of drought resistance in the three seedlings.
出处
《浙江林学院学报》
CSCD
2000年第1期24-27,共4页
Journal of Zhejiang Forestry College
基金
林业部重点攻关资助项目!( 94 0 8 0 2 )
关键词
针叶树
水分胁迫
抗旱性
NR
SOD
生化指标
coniferous trees
water stress
nitrate reductase
superoxide dismutase
drought resistance