摘要
目的 研究脑缺血和再灌流期间的羟自由基变化及氯胺酮对其影响。方法 制作沙土鼠前脑缺血再灌流模型 ,脑缺血 10分钟 ,再灌流 6 0分钟。分为假手术组、缺血组、缺血再灌流组和氯胺酮组。应用高效液相色谱测定纹状体和海马羟自由基 (OH· )、三磷酸腺苷 (ATP)和多巴胺 (DA )含量。结果 海马二羟基苯甲酸 (2 ,3- DHBA)含量缺血组和缺血再灌流组均明显高于假手术组 (P<0 .0 1) ;缺血再灌流组 2 ,3- DHBA的含量明显高于缺血组 (P<0 .0 5 ) ;脑缺血和再灌流期间 ATP和 DA含量均显著降低 (P<0 .0 5 )。氯胺酮可明显增加脑缺血和再灌流期间 ATP和 DA含量 ,减少再灌流期间的 2 ,3- DHBA含量。结论 氯胺酮可能通过抑制脑缺血和再灌流期间 DA的释放和促进 ATP含量的恢复 ,而减少 OH· 的产生 ,这可能是其对脑缺血再灌流损伤起保护作用的机理之一。
Objective To study the change of hydroxyl free radicals during cerebral ischemia and reperfusion and the effect of ketamine on it.Methods Animal model was made by cerebral ischemia for 10 min and reperfusion for 60 min in gerbils. Animals were divided into four groups: sham operative group(SO), ischemic group(I), ischemia reperfusion group(IR) and ketamine treatment group. The striatum,hippocampus hydroxyl free radical(OH ·),ATP content and DA were determined by high performance liquid chromatography(HPLC).Results Hippocampus 2,3 DHBA content in I group and IR group was higher than that in SO group(P<0.01);2,3 DHBA content in IR group was remarkable higher than I group(P<0.05). The contents of ATP and DA were significantly reduced during ischemia and reperfusion(P<0.05). Ketamine (KT) obviously increased the contents of ATP and DA, and reduced the content of 2,3 DHBA during reperfusion.Conclusion KT could reduce OH · generation by inhibiting the release of DA and promoted the recovery of ATP during cerebral ischemia and reperfusion, it might be an important mechanism which had protective effect to cerebral ischemia reperfusion damage.
出处
《临床神经病学杂志》
CAS
2000年第2期70-72,共3页
Journal of Clinical Neurology
基金
江苏省卫生厅自然科学基金资助课题!(H9333)
关键词
沙土鼠
脑缺血
羟自由基
氯胺酮
治疗
Gerbil Cerebral ischemia Hydroxyl free radical Ketamine
