摘要
目的探讨及评价类风湿因子（RF）联合抗环瓜氨酸肽（CCP）抗体和G-6-磷酸酶（GPI）检测在自身类风湿性关节炎（RA）诊断中的意义。方法选择我院2009~2010年收治的RA患者80例（A组）,非RA自身免疫病患者60例（B组）,健康体检者52例（C组）,取空腹血制备血清,采用速率散射免疫比浊法检测RF,以酶联免疫固相吸附试验法（ELISA）检测抗CCP抗体,采用双抗体夹心酶联免疫固相吸附试验法检测血清GPI水平,并分析RF、抗CCP抗体和GPI在诊断RA中的价值。结果 RA患者中RF、抗CCP抗体、GPI的敏感性分别为63.75%,70.00%和83.75%,以GPI最高;三者特异性分别为66.25%、90.00%和91.25%;三项指标联合检测敏感性为96.25%,显著高于任意单项指标（P〈0.05）,且阳性符合率为91.25%。结论抗CCP抗体和GPI可作为RA诊断的实验室有效指标,临床中与RF联合应用可以起到增加敏感性,提高阳性符合率的互补作用。
Objective To explore and evaluate the clinical value of RF combined with CCP and GPI in diagnosis of rheumatoid arthritis.Methods 80 patients with rheumatoid arthritis,60 patients with other autoimmune diseases and 52 normal people were selected and divided into A,B and C group,blood serums were prepared and RF,anti-CCP and GPI were detected by immunization rate nephelometry,enzyme-linked immunosorbent assay and double antibody sandwich enzyme-linked immunosorbent.Results The sensitivity of RF,anti-CCP and GPI in RA patients were 63.75%,70.00% and 83.75% respectively,the GPI was higher than the other index,and the specificity of the three index were 66.25%,90.00% and 91.25% respectively.The sensitivity in combination was 96.25%,which was higher than any single index（P 0.05）,and the positive coincidence rate was 91.25%.Conclusion Anti-CCP and GPI are effective indicators in diagnosis of RA,the two index combination with RF have the effects on improvement of the sensitivity and the positive coincidence rate.
出处
《中国医药导报》
CAS
2012年第12期105-106,共2页
China Medical Herald
关键词
类风湿因子
抗环瓜氨酸肽抗体
葡萄糖-6-磷酸酶
类风湿性关节炎
Rheumatoid factor
Anti-cyclic citrullinated peptide antibodies
Glucose-6-phosphate isomerase
Rheumatoid arthritis
