摘要
目的 研究介入肝动脉栓塞后继发性肝损伤的机制和防护措施。 方法 建立兔肝动脉介入栓塞模型,分组:假手术组(Con),栓塞组(Amb),栓塞+地塞米松治疗组(Dex)和栓塞+前列腺素E治疗组(PGE),于术前、术后1,2,3,6d动态观察其血浆中白介素8(IL-8,ELISA法),内皮素(ET,RIA法)和肝功(ALT,AST,LDH)变化,于术后6d处死动物,观察肝组织学改变, 结果 AMB组术后IL-8水平显著升高,2d时达高峰(2456.68μm/L±613.22μg/L vs 182.83μg/L±49.54μg/L;189.66μg/L±38.76μg/L;368.21μg/L±178.33μg/L,P<0.01),Dex组术后IL-8水平与假手术组无显著差异(189.66μg/L±38.76μg/L;182.83μg/L±49.54μg/L,P>0.05),PGE组术后2d时IL-8水平高于Con组(368±178)μg/L;(183±50)μg/L,(P<0.01),但又明显低于Amb组(P<0.01),Amb,Dex和PGE三组术后血浆ET水平逐渐升高,6d时最高(204±36)μg/L;(232±38)μg/L;(211±29)μg/L vs(146±26)μg/L,P<0.01),三组术后ALT,AST和LDH均显著升高,1d时达高峰[Amb(317±257)U/L,(445±162)U/L,(470±169)U/L,Dex(342±241)U/L,(412±253)U/L,(388±67)U/L和PGE(260±238)U/L,(148±36)U/L,(553±221)U/L vs Con(31±8)U/L,(17±6)U/L,(157±37)U/L,(P<0.01)],至6d时下降接近正常。
AIM To investigate the mechanism and preventive measures of secondary liver injury by hepatic arterial embolization (HAE) in rabbits.METHODS Animal models of HAE were made with lipiodol ultra-fluid. The rabbits were randomly divided into four groups: shamoperation group (Con); hepatic arterial embolization group (Amb); HAE plus dexamethasone group (Dex); and HAE plus prostaglandin E group (PGE). Before and after the operation, the plasma interleukine-8 (IL-8) and endothelin (ET) and serum ALT, AST and LDH were measured by ELISA and RIA. Changes in liver were evaluated histopathologically on the sixth day after the operation.RESULTS On the second day after the operation, plasma IL-8 levels of Amb group (2456.68 μg/L±613.22 μg/L) were significantly higher than those in Con, Dex and PGE groups (182.83 μg/L±49.54 μg/L; 189.66 μg/L± 38.76 μg/L;368.21 μg/L±178.33 μg/L;P<0.01). Plasma IL-8 levels of PGE were higher than those in Con (P<0.05). On the sixth day after the operation, plasma ET levels of Amb, Dex and PGE group rose remarkably than Con group (203.66 μg/L±35.87 μg/L;232.12 μg/L± 38.24 μg/L; 210.68 μg/L ?29.44 μg/L; 146.36 μg/L± 26.22 μg/L, P<0.01). On the first day after the operation, serum ALT, AST and LDH levels of Amb (316.67U/L±256.86 U/L, 444.80 U/L±162.48 U/L, 469.50 U/L±168.70U/L), Dex (342.13U/L± 240.54 U/L, 412.17 U/L±252.88 U/L, 388.20 U/L± 67.29U/L) and PGE ( 259.80U/L± 237.66U/L, 148.00U/L±35.90 U/L,552.60 U/L±221.23 U/L) groups were significantly higher than those in Con (31.22U/L± 7.74 U/L,16.78U/L±5.52 U/L, 156.60 U/L± 36.58 U/L, P<0.01), and they were almost equal to the Con group on the sixth day after the operation. Compared with Amb group, serum ALT and LDH levels dropped remarkably in Dex and PGE group on the second and third days after the operations (P<0.01). Serum AST levels of PGE group were significantly lower than those in Amb group on the first day after the operation. A lot of neutrophils infiltrated around the embolized liver tissue. Liver cells
出处
《世界华人消化杂志》
CAS
2000年第4期413-416,共4页
World Chinese Journal of Digestology
关键词
肝动脉栓塞
肝损伤
IL-8
内皮素
hepatic arterial embolization
rabbit
liver injury
interleukin-8
endothelium
