摘要
目的探讨电针穴位刺激对脑梗死大鼠行为能力及脑梗死侧海马CA1区凋亡相关基因B细胞淋巴瘤／白血病基因-2（Bcl-2）、细胞凋亡通路蛋白X（Bax）表达的影响。方法共选取Wistar雄性成年大鼠48只，根据随机数字表法将其分为电针组及对照组，采用线拴法制成右侧大脑中动脉缺血模型。电针组于造模成功后次日给予电针刺激于百会和大椎穴，每日治疗1次，持续治疗3周；对照组造模成功后常规饲养、自由活动，未给予任何特殊处理。每组分别于制模后第1，2，3周时各取8只大鼠评定其学习记忆行为能力、运动和神经功能，随后断头取脑，采用免疫组织化学法观察2组大鼠梗死侧海马CA1区Bcl-2、Bax蛋白表达量及凋亡指数变化情况。结果各观察时间点电针组大鼠学习记忆行为能力、运动和神经功能均明显优于同期对照组，组问差异均具有统计学意义（P〈0．05）；通过免疫组化检查发现，电针组脑梗死侧海马CAI区Bcl-2蛋白表达在各观察时点均显著高于对照组，而Bax则明显低于对照组，组间差异均具有统计学意义（P〈0．05）；电针组海马CAl区凋亡指数在上述时间点均显著低于对照组（P〈0．05）。结论电针刺激百会、大椎穴可促进脑梗死大鼠行为能力恢复，其治疗机制可能与上调海马CA1区Bcl-2表达、下调Bax表达而抑制缺血半暗带区神经细胞凋亡有关。
Objective To observe effects of electro-acupuncture （EA） on the expression of apoptosis related protein B-cell lymphoma/leukemia gene 2 （Bcl-2） and Bcl-associated protein X （Bax） in the hippocampal CA1 region of rats with cerebral infarction, and on their behavior. Methods An animal model of cerebral ischemia was established by right side middle cerebral artery occlusion using thread in 48 male, adult Wistar rats, who were then randomly divided equally into an EA group and a control group. Both groups were sub-divided into 1 week, 2 weeks and 3 weeks subgroups. The EA group began receiving EA 24h after the occlusion, applied at the Baihui （DU20） and Dazhui （BU14） points, once daily, for one, two or three weeks. The control group was reared conventionally and was not given any special treatment. The ratslearning and memory, motion and neural function were evaluated. The expression of Bcl-2 and Bax in the CA1 region of the hippocampus on the infarcted side and changes of apoptosis in- dexes were detected using immunohistochemical techniques. Results The learning, memory, motion and neural function of the EA group rats were better on average than those of the control group at all observation time points. The expression of Bcl-2 protein increased along with reduced expression of Bax protein in the EA group significantly more than among the controls. TUNEL positive cells in the CA1 region of the hippocampus were reduced significantly in the EA group compared with the control group. Conclusions Behavioral ability after cerebral infarction can be：improved by EA at the Baihui （DU20） and Dazhui （BU14） points. EA can up-regulate the expression of Bcl-2 and down-regulate the expression of Bax and reduce TUNEL positive cells in the CA1 region of the hippocampus after cerebral infarction, which might be the mechanism of its neuroproteetion.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2012年第4期245-249,共5页
Chinese Journal of Physical Medicine and Rehabilitation
基金
四川省中医药专项基金（2010063）
关键词
电针
脑梗死
凋亡相关基因
凋亡
大鼠
Electroacupuncture
Cerebral infarction
Apoptosis
Apoptosis-related genes
