摘要
目的探讨血塞通（XST）干预治疗sD大鼠全脑缺血／再灌注（I／R）后大脑海马回核因子一KappaBp65（NF—κBp65）的表达，阐明其在全脑I／R损伤后的保护作用。方法72只健康SD大鼠，随机分为假手术组（SO）24只，全脑I／R组24只，XST治疗组24只。采用简化的Pulsinelli等的四血管阻塞法建立急性全脑I／R损伤及XST对其作用的模型；用HE染色检测海马CA1区存活锥体细胞数目；用TUNEL原位末端标记法检测锥体细胞凋亡率；用免疫组织化学SABC法检测海马CA1区锥体细胞中NF—KBt,65的表达。结果XST组在再灌注后存活锥体细胞数持续增加，且在用药后3、12、24、48h均明显多于I／R组[（99．23±4．22）个／mmvs（75．83±7．17）个／mm，（80．93±5．36）个／mmvs（51．50±8．26）个／mm，（103．24±5．48）个／mnlvs（35．67±13．17）个／mm，（126．22±7．54）个／mmVS（9．83±4．71）个／mm]，差异均有统计学意义（P〈0．01）；XST组各时间点锥体细胞凋亡率与I／R组比较均明显降低[（8．82±2．71）％VS（22．58±4．68）％，（19．15±6．23）％vs（42．68±3．04）％，（11．82±2．87）％VS（55．51±6．81）％，（8．44±3．23）％vs（71．69±7．71）％]，差异均有统计学意义（P〈0．01）；XST组各时间点NF—κBp65表达阳性的细胞数均明显少于I／R组[（13．204-2．50）VS（18．00±1．87），（8．20±5．31）VS（41．60±3．65），（6．70±3．36）vs（55．30±5．10），（7．10±3．57）VS（72．80±4．71）]，差异均有统计学意义（P〈0．05，P〈0．01）。结论在全脑I／R损伤后，XST可抑制NF—KBp65的表达，抗细胞凋亡，增加存活神经元的数目而起到脑保护作用，从而为XST在脑复苏应用中提供有力的理论依据。
Objective To investigate the expression of NF-κBp65 in hippocampus after the XST intervention therapy in the SD rats with global cerebral I/R injury and testify the protective effect of XST af- ter global cerebral I/R injury. Methods 72 healthy SD rats were randomly divided into 3 groups, sham operation（SO） group （ n =24） , I/R group（ n =24） and XST group（ n =24）. The model of acute global cerebral isehemia/reperfusion （including： I/R and XST group ） injury was produced by means of simple Pulsinellibrierley＇s four arteries occlusion method. H.E. staining was performed to detect the number of surviving neurons and TUNEL was used to detect the rate of neurons apoptosis. The expression activation of NF-κB 1）65 in hippoeampus eornu-ammonis （CA1） region were examined by immunohistoehemieal method （SABC）. Results The survival pymmidal neurons in the XST group continued to increase, and it was significantly more than the I/R group at each time-point after reperfusion[ （99. 23 ±4. 22）/mm vs （75.83 ±7.17）/mm, （80. 93 ± 5.36）/mm vs （51.50 ± 8.26 ）/mm, （ 103.24 ± 5.48 ）/mm vs （35.67 ± 13.17 ）/ mm, （126. 22 ± 7.54 ）/mm vs （9. 83± 4. 71 ）/mm ], the differences were statistically significant （ P 〈 0. 01 ）. The apoptosis rate of pymmidal cell in the XST group at each time-point were more significantly re- duced than the I/R group [ （ 8. 82 ± 2. 71 ） % vs （ 22. 58 ± 4. 68 ） %, （ 19. 15 ±6. 23 ） % vs （ 42. 68 ± 3.04） %,（ 11.82 ± 2. 87 ） % vs （ 55.51 ± 6. 81 ） %, （ 8.44 ± 3.23 ） % vs （ 71.69 ± 7.71 ） % ], the differences were statistically significant （ P 〈 0.01 ）. The positive neurons of NF-KBp65 expression in the XST group at dif- ferent time-points were significantly less than the I/R group[ （ 13.20 ±. 50） vs （ 18.00 ± 1.87）, （8.20 ±5.31 ） vs （41.60 ±.3.65）, （6. 70 ±3.36） vs （55.30 ±5.10）, （7.10 ±3.57） vs （72. 80 ±4. 71 ） ], the differences were statistically sig
出处
《中国医师杂志》
CAS
2012年第3期318-321,共4页
Journal of Chinese Physician
基金
深圳市宝安科技局科研资助项目（20110645）
