摘要
目的采用HPLC技术,阐明中药金莲花中有效成分牡荆苷在体内的药动学及组织分布规律。方法以新西兰白兔为研究对象,通过静脉注射(iv)、腹腔注射(ip)、肌肉注射(im)3种不同给药方式,比较3种不同给药方式的牡荆苷在新西兰白兔体内的药动学特点;研究静脉注射牡荆苷后在兔体内组织分布情况,考察静脉注射牡荆苷后其在动物体内的组织分布特征。采用3P97程序计算药物代谢动力学参数。结果 3种给药方式在兔体内的房室模型均符合二房室开放模型,牡荆苷在新西兰白兔体内分布及消除较快。新西兰白兔静脉注射牡荆苷后,牡荆苷可广泛分布于兔体内各组织中,各组织中AUC大小顺序为肾>肝>肺>脾>心>脑,提示肾对牡荆苷具有很高的摄取率,同时它又较难通过血脑屏障。结论该方法简便、快速、稳定可靠,适用于牡荆苷的体内分析。
AIM To elucidate pharmacokinetics and tissue distribution of active components from Trollius chinensis in vivo by HPLC. METHODS Rabbits were selected to study the pharmacological characteristics of vitexin. Three different kinds of routes of administration including iv, ip, and im, were compared in the tissue distribution by single vein injection, and the pharmacokinetie parameters were analyzed with the 3P97 software. RESULTS The pharmacokinetic characteristics of vitexin presented in rabbits in compliance with the open two-com- partment model in routes of administration and their T1/2β were all relatively quick. Vitexin could distribute widely all over each tissue of rabbits, and the AUC in tissues were measured in turn as follows： kidney 〉 liver 〉 lung 〉 spleen 〉 heart 〉 brain. That showed kidney could eliminate vitexin more rapidly, but was hard to distribute in blood brain. CONCLUSION The method is accurate, reliable with good reproducibility and applicable to pharmacokinetic research on vitexin in vivo.
出处
《中成药》
CAS
CSCD
北大核心
2012年第4期650-653,共4页
Chinese Traditional Patent Medicine
基金
河北省教育厅科学研究计划项目(2008101)
张家口市科技局基金资助项目(0711046D-9)
关键词
金莲花
牡荆苷
HPLC
药动学
组织分布
家兔
Trollius chinensis
vitexin
HPLC
pharmacokinetics
tissue distribution
rabbit
