摘要
目的:建立一种HPLC-ELSD测定复方制剂归芪洋参丸中人参皂苷Rb1含量的方法。方法:采用高效液相色谱-蒸发光散射检测法测定。色谱条件:Diamonsil C1(84.6 mm×250 mm,5μm);流动相乙腈-水(30∶70);流速:1.0 mL/min。ELSD参数:漂移管温度110℃,载气流量2.1 L/min。结果:人参皂苷Rb1在1.832μg^18.32μg范围内呈良好的线性关系,平均回收率为99.70%,RSD=1.90%(n=6)。结论:所建立的方法简便可行,专属性强,重复性好,可用于归芪洋参丸的质量控制。
Objective：To establish the HPLC-ELSD method for determination of ginsenoside Rb1 content in Guiqi Yangshen pills.Method：HPLC-ELSD method was performed on Diamonsil C18 column（4.6 mm×250 mm）with acetonitrile-water（30∶70）as mobile phase,flow rate was at 1 mL/min.ELSD parameters were set as follows：temperature of drift tube was 110 ℃,flow rate of N2 was 2.1 L/min.Result：The linear ranger of ginsenoside Rb1 content was from 1.832 μg to 18.32 μg.The average recovery rate was 99.70%,and RSD was 1.90%（n=6）.Conclusion：The established method is simple,feasible,specific,reproducible,and could be used as the quality control standard of Guiqi Yangshen pills.
出处
《山西中医学院学报》
2012年第1期29-31,共3页
Journal of Shanxi College of Traditional Chinese Medicine
基金
山西省科技厅资助项目(20090321096)
关键词
归芪洋参丸
HPLC
人参皂苷RB1
西洋参
Guiqi Yangshen pills
HPLC
ginsenoside Rb1
radix panacis quinquefolii
